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BRCA1 抗体 (Middle Region)

Name: BRCA1 抗体 (Middle Region)
SKU: ABIN2777874
Availability: OutOfStock

BRCA1 适用: 人, 大鼠, 小鼠, Cow WB, IHC 宿主: 兔 Polyclonal unconjugated

WB Suggested Anti-BRCA1 Antibody Titration: 0. (BRCA1 抗体 (Middle Region))

Western Blot with Cell line 293T (Embyonic human kidney cells) tissue (BRCA1 抗体 (Middle Region))

IHC

2 images

(1 reference)

产品编号 ABIN2777874

发货至: 中国

Datasheet as PDF

抗原 See all BRCA1 抗体

BRCA1 (Breast Cancer 1 (BRCA1))
抗原表位
22

21

15

15

12

12

10

8

7

6

6

5

4

4

3

3

3

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Middle Region
适用
190

33

25

3

2

1

1
人, 大鼠, 小鼠, Cow
宿主
157

38

2
兔
Compatible Secondaries
克隆类型
161

36
多克隆
标记
88

16

9

6

5

5

5

5

5

5

3

3

3

3

3

3

3

3

3

3

3

2

2

2

2

2

2

2

1
This BRCA1 antibody is un-conjugated
应用范围
117

61

42

41

39

34

28

24

23

13

11

10

9

9

3

2

2

2

1

1
Western Blotting (WB), Immunohistochemistry (IHC)

序列

DDLLDDGEIK EDTSFAENDI KESSAVFSKS VQKGELSRSP SPFTHTHLAQ

预测反应

Cow: 75%, Human: 100%, Mouse: 79%, Rat: 86%

产品特性

This is a rabbit polyclonal antibody against BRCA1. It was validated on Western Blot using a cell lysate as a positive control.

纯化方法

Affinity Purified

免疫原

The immunogen is a synthetic peptide directed towards the middle region of human BRCA1

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应用备注

Optimal working dilutions should be determined experimentally by the investigator.

说明

Antigen size: 1863 AA

限制

仅限研究用
状态

Liquid

浓度

Lot specific

缓冲液

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freeze-thaw cycles.

储存条件

-20 °C

储存方法

For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Evans, Shenton, Woodward, Lalloo, Howell, Maher: "Penetrance estimates for BRCA1 and BRCA2 based on genetic testing in a Clinical Cancer Genetics service setting: risks of breast/ovarian cancer quoted should reflect the cancer burden in the family." in: BMC cancer, Vol. 8, pp. 155, (2008) (PubMed).
抗原

BRCA1 (Breast Cancer 1 (BRCA1))

别名

BRCA1 (BRCA1 产品)

背景

The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. BRCA1 acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. BRCA1 plays a central role in DNA repair by facilitating cellular response to DNA repair. BRCA1 is required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. BRCA1 is involved in transcriptional regulation of P21 in response to DNA damage. BRCA1 is also required for FANCD2 targeting to sites of DNA damage.It may function as a transcriptional regulator. BRCA1 inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation.This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability and acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as BASC for BRCA1-associated genome surveillance complex. This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complex. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40 % of inherited breast cancers and more than 80 % of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants have been described for this gene but only some have had their full-length natures identified.
Alias Symbols: BRCAI, BRCC1, IRIS, PSCP, RNF53, PNCA4, BROVCA1, PPP1R53
Protein Interaction Partner: RAD50, MED17, RAD51, YAP3, HDA3, SSN3, BEM4, GYP5, YAF9, HDAC2, MSH6, BAP1, SUPT5H, POLR2A, NPR1, NUP53, MET18, CDC21, MLP1, CTK1, MSH2, BACH1, DAN1, TMA22, BBC1, RPB4, SPT4, YGR053C, ATE1, YBP2, SLI15, RAD16, XRS2, RAD55, BUR2, BRIP1, ESR1, BECN1, HIST1H
Protein Size: 1863

分子量

208 kDa

基因ID

672

NCBI登录号

NM_007294, NP_009225

UniProt

P38398

途径

Cell Division Cycle, DNA Damage Repair, Intracellular Steroid Hormone Receptor Signaling Pathway, Positive Regulation of Response to DNA Damage Stimulus