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GCLC 抗体 (N-Term)

Name: GCLC 抗体 (N-Term)
SKU: ABIN2774078
Availability: OutOfStock

GCLC 适用: 人, 小鼠, 大鼠, Cow, 斑马鱼, 兔, 犬, 豚鼠, 马 WB, IHC 宿主: 兔 Polyclonal unconjugated

WB Suggested Anti-GCLC Antibody Titration: 0. (GCLC 抗体 (N-Term))

GCLC antibody - N-terminal region (ARP54576_P050) Catalog Number: ARP54576_P050 Formalin Fixed Paraffin Embedded Tissue: Human Bronchial Epithelial Tissue Observed Staining: Cytoplasm and membrane of bronchial epithelial tissue Primary Antibody Concentration: 1:600 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0. (GCLC 抗体 (N-Term))

IHC

Host: Rabbit Target Name: GCLC Sample Tissue: Mouse Heart Antibody Dilution: 1ug/ml (GCLC 抗体 (N-Term))

Lanes: 1: 40ug mouse heart lysate, 2: 40ug mouse heart lysate, 3: 40ug mouse heart lysate, 4: 40ug mouse heart lysate, 5: 40ug mouse heart lysate Primary Antibody Dilution: 1:1000 Secondary Antibody: Anti-rabbit HRP Secondary Antibody Dilution: 1:10000 Gene Name: GCLC Submitted by: Anonymous (GCLC 抗体 (N-Term))

5 images

(1 reference)

产品编号 ABIN2774078

发货至: 中国

Datasheet as PDF

抗原 See all GCLC 抗体

GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))
抗原表位
9

8

6

6

4

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1
N-Term
适用
59

21

19

4

3

3

2

2

2

2

1

1

1

1

1

1

1

1
人, 小鼠, 大鼠, Cow, 斑马鱼, 兔, 犬, 豚鼠, 马
宿主
59

4
兔
Compatible Secondaries
克隆类型
50

13
多克隆
标记
37

4

3

3

2

2

2

2

2

2

2

2
This GCLC antibody is un-conjugated
应用范围
45

26

21

13

11

10

9

5

2

1

1
Western Blotting (WB), Immunohistochemistry (IHC)

序列

VLETLQEKGE RTNPNHPTLW RPEYGSYMIE GTPGQPYGGT MSEFNTVEAN

预测反应

Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 79%

产品特性

This is a rabbit polyclonal antibody against GCLC. It was validated on Western Blot using a cell lysate as a positive control.

纯化方法

Affinity Purified

免疫原

The immunogen is a synthetic peptide directed towards the N terminal region of human GCLC

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anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (AA 437-637) antibody
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anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (AA 1-30), (N-Term) antibody
GCLC 适用: 人 WB, IF, IHC (p), FACS 宿主: 兔 Polyclonal RB31687 unconjugated

anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (AA 1-252) antibody
GCLC 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (AA 435-636) antibody
GCLC 适用: 大鼠 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated

anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (Middle Region) antibody
GCLC 适用: 人, 小鼠, 大鼠, Cow, 斑马鱼, 兔, 犬, 豚鼠, 马, Saccharomyces cerevisiae WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (AA 6-34), (N-Term) antibody
GCLC 适用: 人 WB, IF, IHC (p), FACS, EIA 宿主: 兔 Polyclonal unconjugated

anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (AA 435-636) antibody
GCLC 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

Optimal working dilutions should be determined experimentally by the investigator.

说明

Antigen size: 637 AA

限制

仅限研究用
状态

Liquid

浓度

Lot specific

缓冲液

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freeze-thaw cycles.

储存条件

-20 °C

储存方法

For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Tomasi, Ryoo, Yang, Iglesias Ara, Ko, Lu: "Molecular mechanisms of lipopolysaccharide-mediated inhibition of glutathione synthesis in mice." in: Free radical biology & medicine, Vol. 68, pp. 148-58, (2014) (PubMed).
抗原

GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

别名

GCLC (GCLC 产品)

背景

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. GCLC is the catalytic subunit of 637 amino acids with a calculated molecular weight of 72.773 kDa. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia.Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. The gene encoding the catalytic subunit encodes a protein of 367 amino acids with a calculated molecular weight of 72.773 kDa and maps to chromosome 6. The regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: GCS, GLCL, GLCLC, GCL
Protein Interaction Partner: UBC, PPID, GCLM, CCL22, PAXIP1, ELAVL1,
Protein Size: 637

分子量

73 kDa

基因ID

2729

NCBI登录号

NM_001498, NP_001489

UniProt

P48506

途径

Cell RedoxHomeostasis