(6 references)-
- 抗原 See all CD82 抗体
- CD82
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD82 antibody is conjugated to APC
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应用范围
- Flow Cytometry (FACS)
- 特异性
- The mouse monoclonal antibody C33 recognizes an extracellular/luminal epitope of CD82, a widely expressed cell surface protein of the tetraspanin family. CD82 is also found in endosome/lysosome compartments.
- 交叉反应 （详细）
- Human, Other not tested
- 纯化方法
- Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
- 免疫原
- C91/PL (human HTLV-1+ T cell line)
- 克隆位点
- C33
- 亚型
- IgG2a
- Top Product
- Discover our top product CD82 Primary Antibody
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anti-CD82 (CD82) antibody
CD82 适用: 人 WB, FACS, IHC (p), IP, ICC 宿主: 小鼠 Monoclonal C33 unconjugated
anti-CD82 (CD82) antibodyCD82 适用: 人 WB, FACS, IHC (p), IP, ICC, Func 宿主: 小鼠 Monoclonal C33 unconjugated
anti-CD82 (CD82) antibodyCD82 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-CD82 (CD82) (AA 239-267), (C-Term) antibodyCD82 适用: 人, 小鼠 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB02073-02074 unconjugated
anti-CD82 (CD82) antibodyCD82 适用: 人 FACS, IHC 宿主: 小鼠 Monoclonal B-L2 unconjugated
anti-CD82 (CD82) (AA 98-267) antibodyCD82 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-CD82 (CD82) (AA 111-228) antibodyCD82 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD82 (CD82) (AA 1-267) antibodyCD82 适用: 人 ELISA, PLA 宿主: 小鼠 Monoclonal 4F2 unconjugated
anti-CD82 (CD82) antibody (FITC)CD82 适用: 人 FACS 宿主: 小鼠 Monoclonal C33 FITC
anti-CD82 (CD82) antibody (Biotin)CD82 适用: 人 WB, FACS, IHC (p), IP, ICC 宿主: 小鼠 Monoclonal C33 Biotin
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- 应用备注
- Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
- 说明
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The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
- 限制
- 仅限研究用
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- 缓冲液
- Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
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: "Rapid multiplex analysis of lipid raft components with single-cell resolution." in: Science signaling, Vol. 8, Issue 395, pp. rs11, (2015) (PubMed).
: "Selective enrichment of tetraspan proteins on the internal vesicles of multivesicular endosomes and on exosomes secreted by human B-lymphocytes." in: The Journal of biological chemistry, Vol. 273, Issue 32, pp. 20121-7, (1998) (PubMed).
: "Expression of the KAI1 protein in benign prostatic hyperplasia and prostate cancer." in: The American journal of pathology, Vol. 149, Issue 5, pp. 1435-40, (1996) (PubMed).
: "Molecular analyses of the association of CD4 with two members of the transmembrane 4 superfamily, CD81 and CD82." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 3, pp. 1229-39, (1995) (PubMed).
: "C33 antigen and M38 antigen recognized by monoclonal antibodies inhibitory to syncytium formation by human T cell leukemia virus type 1 are both members of the transmembrane 4 superfamily and ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 11, pp. 6470-81, (1994) (PubMed).
: "Identification of membrane antigen C33 recognized by monoclonal antibodies inhibitory to human T-cell leukemia virus type 1 (HTLV-1)-induced syncytium formation: altered glycosylation of C33 antigen ..." in: Journal of virology, Vol. 66, Issue 3, pp. 1394-401, (1992) (PubMed).
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: "Rapid multiplex analysis of lipid raft components with single-cell resolution." in: Science signaling, Vol. 8, Issue 395, pp. rs11, (2015) (PubMed).
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- 抗原
- CD82
- 别名
- CD82 (CD82 产品)
- 别名
- AA682076 antibody, AL023070 antibody, C33 antibody, IA4 antibody, Kai1 antibody, Tspan27 antibody, 4F9 antibody, GR15 antibody, KAI1 antibody, R2 antibody, SAR2 antibody, ST6 antibody, TSPAN27 antibody, CD82 antigen antibody, CD82 molecule antibody, Cd82 molecule antibody, Cd82 antibody, CD82 antibody
- 背景
- CD82 Molecule,CD82 (KAI1), a member of the tetraspanin family, forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules. These complexes influence adhesion, morphology, activation, proliferation and differentiation of B, T and other cells. CD82 regulates cytoskeleton rearrangement and may participate in the turnover of the tetraspanin complex members. Besides in the plasma membrane, CD82 is localized also in endosome/lysosome compartments. Tumour-suppressive roles of CD82 have been demonstrated.,R2, 4F9, C33, IA4, ST6, GR15, KAI1, SAR2, TSPAN27
- 基因ID
- 3732
- UniProt
- P27701
- 途径
- p53 Pathway
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