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CD82 抗体

Name: CD82 抗体
Brand: antibodies-online
SKU: ABIN2749189
Availability: OutOfStock

CD82 适用: 人 WB, FACS, IHC (p), IP, ICC, Func 宿主: 小鼠 Monoclonal C33 unconjugated

Separation of human CD82 positive lymphocytes (red-filled) from CD82 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD82 (C33) purified antibody (low endotoxin, concentration in sample 1 μg/mL) GAM APC. (CD82 抗体)

FACS

Immunohistochemistry staining of human tonsil (paraffin-embedded sections) with anti-CD82 (C33). (CD82 抗体)

IHC (p)

Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD82 (C33) purified antibody (low endotoxin, concentration in sample 1 μg/mL) GAM APC. (CD82 抗体)

FACS

3 images

(6 references)

产品编号 ABIN2749189

发货至: 中国

Datasheet as PDF

抗原 See all CD82 抗体

CD82
适用
59

42

6

1

1

1
人
宿主
60

26
小鼠
Compatible Secondaries
克隆类型
59

27
单克隆
标记
30

9

7

5

4

4

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1
This CD82 antibody is un-conjugated
应用范围
52

32

24

20

19

18

13

13

7

6

6

5

3

2

1

1
Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC), Functional Studies (Func)

特异性

The mouse monoclonal antibody C33 recognizes an extracellular/luminal epitope of CD82, a widely expressed cell surface protein of the tetraspanin family. CD82 is also found in endosome/lysosome compartments.

交叉反应（详细）

Human, Other not tested

纯化方法

Purified by protein-A affinity chromatography.

纯度

> 95 % (by SDS-PAGE)

内毒素水平

Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

免疫原

C91/PL (human HTLV-1+ T cell line)

克隆位点

C33

亚型

IgG2a

Top Product

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anti-CD82 (CD82) antibody
CD82 适用: 人 WB, FACS, IHC (p), IP, ICC 宿主: 小鼠 Monoclonal C33 unconjugated

anti-CD82 (CD82) antibody
CD82 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated

anti-CD82 (CD82) (AA 239-267), (C-Term) antibody
CD82 适用: 人, 小鼠 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB02073-02074 unconjugated

anti-CD82 (CD82) antibody
CD82 适用: 人 FACS, IHC 宿主: 小鼠 Monoclonal B-L2 unconjugated

anti-CD82 (CD82) (AA 98-267) antibody
CD82 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-CD82 (CD82) (AA 111-228) antibody
CD82 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-CD82 (CD82) (AA 1-267) antibody
CD82 适用: 人 ELISA, PLA 宿主: 小鼠 Monoclonal 4F2 unconjugated

anti-CD82 (CD82) antibody (FITC)
CD82 适用: 人 FACS 宿主: 小鼠 Monoclonal C33 FITC

anti-CD82 (CD82) antibody (Biotin)
CD82 适用: 人 WB, FACS, IHC (p), IP, ICC 宿主: 小鼠 Monoclonal C33 Biotin

anti-CD82 (CD82) antibody (APC)
CD82 适用: 人 FACS 宿主: 小鼠 Monoclonal C33 APC

应用备注

Functional application: In human MOLT-4 T-cell line the antibody C33 inhibits syncytium formation induced by coculture with human T-cell leukemia virus type 1 (HTLV-1)-positive human T-cell lines.
Flow cytometry: Recommended dilution: 1-4 μg/mL

限制

仅限研究用
浓度

1 mg/mL

缓冲液

Phosphate buffered saline (PBS), pH 7.4

储存液

Azide free

储存条件

4 °C

储存方法

Store at 2-8°C. Do not freeze.
Schatzlmaier, Supper, Göschl, Zwirzitz, Eckerstorfer, Ellmeier, Huppa, Stockinger: "Rapid multiplex analysis of lipid raft components with single-cell resolution." in: Science signaling, Vol. 8, Issue 395, pp. rs11, (2015) (PubMed).

Escola, Kleijmeer, Stoorvogel, Griffith, Yoshie, Geuze: "Selective enrichment of tetraspan proteins on the internal vesicles of multivesicular endosomes and on exosomes secreted by human B-lymphocytes." in: The Journal of biological chemistry, Vol. 273, Issue 32, pp. 20121-7, (1998) (PubMed).

Ueda, Ichikawa, Tamaru, Mikata, Akakura, Akimoto, Imai, Yoshie, Shiraishi, Yatani, Ito, Shimazaki: "Expression of the KAI1 protein in benign prostatic hyperplasia and prostate cancer." in: The American journal of pathology, Vol. 149, Issue 5, pp. 1435-40, (1996) (PubMed).

Imai, Kakizaki, Nishimura, Yoshie: "Molecular analyses of the association of CD4 with two members of the transmembrane 4 superfamily, CD81 and CD82." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 3, pp. 1229-39, (1995) (PubMed).

Imai, Yoshie et al.: "C33 antigen and M38 antigen recognized by monoclonal antibodies inhibitory to syncytium formation by human T cell leukemia virus type 1 are both members of the transmembrane 4 superfamily and ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 11, pp. 6470-81, (1994) (PubMed).

Fukudome, Furuse, Imai, Nishimura, Takagi, Hinuma, Yoshie et al.: "Identification of membrane antigen C33 recognized by monoclonal antibodies inhibitory to human T-cell leukemia virus type 1 (HTLV-1)-induced syncytium formation: altered glycosylation of C33 antigen ..." in: Journal of virology, Vol. 66, Issue 3, pp. 1394-401, (1992) (PubMed).
抗原

CD82

别名

CD82 (CD82 产品)

别名

AA682076 antibody, AL023070 antibody, C33 antibody, IA4 antibody, Kai1 antibody, Tspan27 antibody, 4F9 antibody, GR15 antibody, KAI1 antibody, R2 antibody, SAR2 antibody, ST6 antibody, TSPAN27 antibody, CD82 antigen antibody, CD82 molecule antibody, Cd82 molecule antibody, Cd82 antibody, CD82 antibody

背景

CD82 Molecule,CD82 (KAI1), a member of the tetraspanin family, forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules. These complexes influence adhesion, morphology, activation, proliferation and differentiation of B, T and other cells. CD82 regulates cytoskeleton rearrangement and may participate in the turnover of the tetraspanin complex members. Besides in the plasma membrane, CD82 is localized also in endosome/lysosome compartments. Tumour-suppressive roles of CD82 have been demonstrated.,R2, 4F9, C33, IA4, ST6, GR15, KAI1, SAR2, TSPAN27

基因ID

3732

UniProt

P27701

途径

p53 Pathway