Ki-67 抗体
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- 抗原 See all Ki-67 (MKI67) 抗体
- Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))
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适用
- 人, Cow
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Ki-67 antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC)
- 特异性
- The mouse monoclonal antibody Ki-67 recognizes Ki-67 antigen, a non-histone nuclear protein expressed exclusively in proliferating cells.
- 交叉反应 (详细)
- Human, Bovine
- 纯化方法
- Purified by protein-A affinity chromatography.
- 纯度
- > 95 % (by SDS-PAGE)
- 免疫原
- Nuclei of the Hodgkin lymphoma cell line L428
- 克隆位点
- Ki-67
- 亚型
- IgG1
- Top Product
- Discover our top product MKI67 Primary Antibody
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- 应用备注
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Flow cytometry: Recommended dilution: 1-5 μg/mL. Intracellular staining.
Immunocytochemistry: Paraformaldehyde fixation, recommended antibody concentration 1 μg/mL. - 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store at 2-8°C. Do not freeze.
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The cell proliferation-associated antigen of antibody Ki-67: a very large, ubiquitous nuclear protein with numerous repeated elements, representing a new kind of cell cycle-maintaining proteins." in: The Journal of cell biology, Vol. 123, Issue 3, pp. 513-22, (1993) (PubMed).
: "Cell cycle analysis of a cell proliferation-associated human nuclear antigen defined by the monoclonal antibody Ki-67." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 133, Issue 4, pp. 1710-5, (1984) (PubMed).
: "Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation." in: International journal of cancer. Journal international du cancer, Vol. 31, Issue 1, pp. 13-20, (1983) (PubMed).
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The cell proliferation-associated antigen of antibody Ki-67: a very large, ubiquitous nuclear protein with numerous repeated elements, representing a new kind of cell cycle-maintaining proteins." in: The Journal of cell biology, Vol. 123, Issue 3, pp. 513-22, (1993) (PubMed).
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- 抗原
- Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))
- 别名
- Ki-67 (MKI67 产品)
- 别名
- MKI67 antibody, kia antibody, mki67 antibody, MGC132156 antibody, D630048A14Rik antibody, Ki-67 antibody, Ki67 antibody, KIA antibody, MIB-1 antibody, marker of proliferation Ki-67 antibody, marker of proliferation Ki-67 L homeolog antibody, marker of proliferation Ki-67 S homeolog antibody, antigen identified by monoclonal antibody Ki 67 antibody, antigen identified by monoclonal antibody Ki-67 antibody, nucleolar protein interacting with the FHA domain of MKI67 antibody, MKI67 antibody, mki67 antibody, mki67.L antibody, mki67.S antibody, Mki67 antibody, NIFK antibody
- 背景
- Marker of proliferation Ki-67,Ki-67 is a highly protease-sensitive nuclear protein expressed in two isoforms (345 kDa and 395 kDa), both of which are identified by the antibody clone Ki-67. The Ki-67 antigen is essential for cell proliferation and its expression is restricted to the cycling cells. It is detected in G1, S, G2 and M phase, whereas it is absent in cells which are in G0 phase and it is not associated with DNA repair processes. Ki-67 thus represents an important tool for detection of proliferating cells, which is of great importance in tumor diagnostics and is commonly used as a prognostic factor in cancer studies.,KIA, MIB, Marker of proliferation Ki67, MKI67
- 基因ID
- 4288
- UniProt
- P46013
- 途径
- Glycosaminoglycan Metabolic Process
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