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CD40 Ligand 抗体

Name: CD40 Ligand 抗体
Brand: antibodies-online
SKU: ABIN2749183
Availability: OutOfStock

CD40LG 适用: 人, Non-Human Primate FACS, ICC 宿主: 小鼠 Monoclonal 24-31 unconjugated

Flow cytometry surface staining pattern of human stimulated (PMA + ionomycin) peripheral blood mononuclear cells stained using anti-human CD154 (24-31) purified antibody (concentration in sample 2 μg/mL) GAM APC. (CD40 Ligand 抗体)

FACS

Separation of human CD154 positive cells (red-filled) from CD154 negative cells (black-dashed) in flow cytometry analysis (surface staining) of stimulated (PMA + ionomycin) peripheral blood mononuclear cells stained using anti-human CD154 (24-31) purified antibody (concentration in sample 2 μg/mL) GAM APC. (CD40 Ligand 抗体)

FACS

2 images

(5 references)

产品编号 ABIN2749183

发货至: 中国

Datasheet as PDF

抗原 See all CD40 Ligand (CD40LG) 抗体

CD40 Ligand (CD40LG)
适用
152

81

34

9

8

6

4

2

2

2

2

1

1

1

1

1
人, Non-Human Primate
宿主
97

65

22

15

12

1
小鼠
Compatible Secondaries
克隆类型
112

94

4
单克隆
标记
105

23

21

14

8

3

3

3

3

3

3

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD40 Ligand antibody is un-conjugated
应用范围
109

101

53

51

39

28

20

17

15

14

13

13

5

4

4

4

3

3

2

2

2

1

1

1

1

1

1
Flow Cytometry (FACS), Immunocytochemistry (ICC)

特异性

The mouse monoclonal antibody 24-31 detects an extracellular epitope of CD154 / CD40L (CD40-ligand), a 39 kDa cell surface type II glycoprotein expressed predominantly on activated CD4+ lymphocytes.

交叉反应（详细）

Human, Non-Human Primates

纯化方法

Purified by protein-A affinity chromatography.

纯度

> 95 % (by SDS-PAGE)

免疫原

human CD154 fusion protein

克隆位点

24-31

亚型

IgG1

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anti-CD40 Ligand (CD40LG) (AA 108-261) antibody
CD40LG 适用: 人 FACS, IHC, ELISA, IF, Coat, StM 宿主: 小鼠 Monoclonal CD40LG-2761 unconjugated

anti-CD40 Ligand (CD40LG) antibody (FITC)
CD40LG 适用: 小鼠 FACS 宿主: Armenian Hamster Monoclonal MR-1 FITC

anti-CD40 Ligand (CD40LG) (AA 79-129) antibody
CD40LG 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-CD40 Ligand (CD40LG) (AA 33-62) antibody
CD40LG 适用: 人 WB, FACS, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-CD40 Ligand (CD40LG) (AA 111-261) antibody
CD40LG 适用: Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-CD40 Ligand (CD40LG) antibody (PE)
CD40LG 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal 24-31 PE

anti-CD40 Ligand (CD40LG) (AA 47-261) antibody
CD40LG 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 8H10F5 unconjugated

anti-CD40 Ligand (CD40LG) (AA 47-261) antibody
CD40LG 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 5A3A9 unconjugated

应用备注

Flow cytometry: Recommended dilution: 2-6 μg/mL.

限制

仅限研究用
浓度

1 mg/mL

缓冲液

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C

储存方法

Store at 2-8°C. Do not freeze.
Brams, Black, Padlan, Hariharan, Leonard, Chambers-Slater, Noelle, Newman: "A humanized anti-human CD154 monoclonal antibody blocks CD154-CD40 mediated human B cell activation." in: International immunopharmacology, Vol. 1, Issue 2, pp. 277-94, (2001) (PubMed).

Berner, Wolf, Hummel, Müller, Reuss-Borst: "Increased expression of CD40 ligand (CD154) on CD4+ T cells as a marker of disease activity in rheumatoid arthritis." in: Annals of the rheumatic diseases, Vol. 59, Issue 3, pp. 190-5, (2000) (PubMed).

Barnhart, Ford, Bhushan, Song, Covey: "A polymorphic CD40 ligand (CD154) molecule mediates CD40-dependent signalling but interferes with the ability of soluble CD40 to functionally block CD154:CD40 interactions." in: Immunology, Vol. 99, Issue 1, pp. 54-61, (2000) (PubMed).

Grammer, McFarland, Heaney, Darnell, Lipsky: "Expression, regulation, and function of B cell-expressed CD154 in germinal centers." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 8, pp. 4150-9, (1999) (PubMed).

Kornbluth, Kee, Richman: "CD40 ligand (CD154) stimulation of macrophages to produce HIV-1-suppressive beta-chemokines." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 9, pp. 5205-10, (1998) (PubMed).
抗原

CD40 Ligand (CD40LG)

别名

CD154 / CD40L (CD40LG 产品)

别名

CD154 antibody, cd40l antibody, CD40L antibody, HIGM1 antibody, IGM antibody, IMD3 antibody, T-BAM antibody, TNFSF5 antibody, TRAP antibody, gp39 antibody, hCD40L antibody, CD40-L antibody, Cd40l antibody, Ly-62 antibody, Ly62 antibody, Tnfsf5 antibody, CD40 ligand antibody, TNF superfamily member 5 antibody, CD40LG antibody, cd40l antibody, Cd40lg antibody

背景

CD40 ligand,CD154 / CD40L (CD40 ligand) is a member of the tumor necrosis factor family, and is expressed primarily on activated CD4+ lymphocytes, but also on mast cells, basophils, eosinophils and human dendritic cells. Its counter-receptor CD40 is expressed on antigen presenting cells, including dendritic cells, macrophages, and B cells, and also on fibroblasts. Triggering of CD40 by CD40L causes maturation of dendritic cells and upregulation of antigen presentation in functions of the MHC and costimulatory molecules. CD40L also functions as a direct stimulating factor for T cells. CD40L plays also roles e.g. in antibody class switching and modulation of apoptosis in the germinal center.,CD40L, CD40 ligand, TNFSF5, Ly62, gp39, T-BAM

基因ID

21947

UniProt

P27548

途径

NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints