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HLA-DR 抗体 (PE)

Name: HLA-DR 抗体 (PE)
Brand: antibodies-online
SKU: ABIN2749150
Availability: OutOfStock

HLA-DR 适用: 人, 犬, Non-Human Primate FACS 宿主: 小鼠 Monoclonal L243 PE

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human HLA-DR (L243) PE antibody (10 μL reagent / 100 μL of peripheral whole blood). (HLA-DR 抗体 (PE))

FACS

Flow cytometry multicolor surface staining pattern of human lymphocytes using anti-human CD3 (UCHT1) APC antibody (10 μL reagent / 100 μL of peripheral whole blood) and anti-human HLA-DR (L243) PE antibody (10 μL reagent / 100 μL of peripheral whole blood). (HLA-DR 抗体 (PE))

FACS

Separation of human CD3 negative HLA-DR positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human HLA-DR (L243) PE antibody (10 μL reagent / 100 μL of peripheral whole blood). (HLA-DR 抗体 (PE))

FACS

3 images

(7 references)

产品编号 ABIN2749150

发货至: 中国

Datasheet as PDF

抗原 See all HLA-DR products

HLA-DR
适用
120

20

19

18

18

14

11

8

5

4

3

3

2
人, 犬, Non-Human Primate
宿主
101

13

9
小鼠
Compatible Secondaries
克隆类型
116

6
单克隆
标记
43

15

13

9

9

4

3

3

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This HLA-DR antibody is conjugated to PE
应用范围
107

33

24

20

15

10

10

9

6

6

3

2

2

2

1

1

1

1

1

1
Flow Cytometry (FACS)

特异性

The mouse monoclonal antibody L243 recognizes specifically an extracellular epitope on HLA-DR molecules, both peptide-loaded and empty.

交叉反应（详细）

Human, Non-Human Primates, Canine (Dog)

纯化方法

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

免疫原

Human B lymphocytes

克隆位点

L243

亚型

IgG2a kappa
anti-HLA-DR (HLA-DR) antibody
HLA-DR 适用: 人 FACS, WB, IHC, ISt, StM 宿主: 小鼠 Monoclonal TAL 1B5 unconjugated

anti-HLA-DR (HLA-DR) antibody (FITC)
HLA-DR 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-12 FITC

anti-HLA-DR (HLA-DR) antibody
HLA-DR 适用: 人 FACS, WB, IHC, IF, ISt, StM 宿主: 小鼠 Monoclonal SPM289 unconjugated

anti-HLA-DR (HLA-DR) antibody
HLA-DR 适用: 人 FACS, WB, IP 宿主: 小鼠 Monoclonal MEM-12 unconjugated

anti-HLA-DR (HLA-DR) antibody
HLA-DR 适用: 人, 犬, Non-Human Primate FACS, WB, IP, IHC (fro), IHC (p), ICC 宿主: 小鼠 Monoclonal L243 unconjugated

anti-HLA-DR (HLA-DR) antibody
HLA-DR 适用: 人 FACS, IP 宿主: 小鼠 Monoclonal HL-39 unconjugated

anti-HLA-DR (HLA-DR) antibody
HLA-DR 适用: 人, 犬, Non-Human Primate FACS, WB, IP, IHC (fro), IHC (p), ICC, Func 宿主: 小鼠 Monoclonal L243 unconjugated

anti-HLA-DR (HLA-DR) antibody (PE)
HLA-DR 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-12 PE

anti-HLA-DR (HLA-DR) antibody (APC)
HLA-DR 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-12 APC

anti-HLA-DR (HLA-DR) antibody (PerCP)
HLA-DR 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-12 PerCP

应用备注

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

说明

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

限制

仅限研究用
缓冲液

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C

储存方法

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Ivanov, Beers, Walshe, Honeychurch, Alduaij, Cox, Potter, Murray, Chan, Klymenko, Erenpreisa, Glennie, Illidge, Cragg: "Monoclonal antibodies directed to CD20 and HLA-DR can elicit homotypic adhesion followed by lysosome-mediated cell death in human lymphoma and leukemia cells." in: The Journal of clinical investigation, Vol. 119, Issue 8, pp. 2143-59, (2009) (PubMed).

De Gassart, Camosseto, Thibodeau, Ceppi, Catalan, Pierre, Gatti: "MHC class II stabilization at the surface of human dendritic cells is the result of maturation-dependent MARCH I down-regulation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 9, pp. 3491-6, (2008) (PubMed).

Swiatek-de Lange, Rist, Stahl, Weith, Lenter: "Comment on "MHC class II expression identifies functionally distinct human regulatory T cells"." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 6, pp. 3625; author reply 3626, (2008) (PubMed).

Muczynski, Ekle, Coder, Anderson: "Normal human kidney HLA-DR-expressing renal microvascular endothelial cells: characterization, isolation, and regulation of MHC class II expression." in: Journal of the American Society of Nephrology : JASN, Vol. 14, Issue 5, pp. 1336-48, (2003) (PubMed).

Bouillon, El Fakhry, Girouard, Khalil, Thibodeau, Mourad: "Lipid raft-dependent and -independent signaling through HLA-DR molecules." in: The Journal of biological chemistry, Vol. 278, Issue 9, pp. 7099-107, (2003) (PubMed).

Kalka-Moll, Tzianabos, Bryant, Niemeyer, Ploegh, Kasper: "Zwitterionic polysaccharides stimulate T cells by MHC class II-dependent interactions." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 169, Issue 11, pp. 6149-53, (2002) (PubMed).

Coral, Pucillo, Leonardi, Fonsatti, Altomonte, Maio: "Triggering of HLA-DR antigens differentially modulates tumor necrosis factor alpha release by B cells at distinct stage of maturation." in: Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research, Vol. 8, Issue 5, pp. 581-8, (1997) (PubMed).
抗原

HLA-DR

别名

HLA-DR (HLA-DR 产品)

背景

HLA-DR, a member of MHC class II glycoproteins, that bind intracellularly processed peptides and present them to the Th cells, is composed of 36 kDa alpha chain and 27 kDa beta chain, both anchored in the plasma membrane. Together with other MHC II molecules HLA-DR plays a central role in the immune system.

途径

Human Leukocyte Antigen (HLA) in Adaptive Immune Response