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Fc gamma RII (CD32) 抗体 (PE)

Name: Fc gamma RII (CD32) 抗体 (PE)
Brand: antibodies-online
SKU: ABIN2749126
Availability: OutOfStock

CD32 适用: 人 FACS 宿主: 小鼠 Monoclonal 3D3 PE

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD32 (3D3) PE antibody (10 μL reagent / 100 μL of peripheral whole blood). (Fc gamma RII (CD32) 抗体 (PE))

FACS

Surface staining of human peripheral blood with anti-human CD32 (3D3) PE. (Fc gamma RII (CD32) 抗体 (PE))

FACS

Separation of human CD32 positive lymphocytes (red-filled) from CD32 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD32 (3D3) PE antibody (10 μL reagent / 100 μL of peripheral whole blood). (Fc gamma RII (CD32) 抗体 (PE))

FACS

3 images

(5 references)

产品编号 ABIN2749126

发货至: 中国

Datasheet as PDF

抗原 See all Fc gamma RII (CD32) 抗体

Fc gamma RII (CD32)
适用
120

9

8

7

5

5

5

3
人
宿主
69

60
小鼠
Compatible Secondaries
克隆类型
66

60

3
单克隆
标记
44

11

10

7

5

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1
PE
应用范围
69

54

23

20

12

11

8

7

6

3

2

2

1

1

1

1
Flow Cytometry (FACS)

特异性

The mouse monoclonal antibody 3D3 recognizes an extracellular epitope of CD32, a 40 kDa polymorphic transmembrane glycoprotein serving as the low affinity receptor for aggregated IgG. This antibody recognizes CD32 isoforms on B cells of all donors, but on platelets, monocytes, and granulocytes of only some donors (131R variant, but not 131H variant).

交叉反应（详细）

Human

纯化方法

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

免疫原

purified glycosylated recombinant human FcgammaRIIa2

克隆位点

3D3

亚型

IgG1 kappa

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anti-Fc gamma RII (CD32) (AA 201-300) antibody
CD32 适用: 人 FACS, WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Fc gamma RII (CD32) antibody
CD32 适用: 人 FACS, CyTOF 宿主: 小鼠 Monoclonal 3D3 unconjugated

anti-Fc gamma RII (CD32) (AA 69-309) antibody
CD32 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Fc gamma RII (CD32) antibody (PE)
GMP Grade CD32 适用: 人 FACS 宿主: 小鼠 Monoclonal IV-3 PE

anti-Fc gamma RII (CD32) antibody (FITC)
GMP Grade CD32 适用: 人 FACS 宿主: 小鼠 Monoclonal IV-3 FITC

anti-Fc gamma RII (CD32) (AA 36-229) antibody
CD32 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Fc gamma RII (CD32) antibody (FITC)
CD32 适用: 人 FACS 宿主: 小鼠 Monoclonal 3D3 FITC

anti-Fc gamma RII (CD32) antibody (APC)
CD32 适用: 人 FACS 宿主: 小鼠 Monoclonal 3D3 APC

anti-Fc gamma RII (CD32) antibody (FITC)
CD32 适用: 人 FACS 宿主: 小鼠 Monoclonal 4AIV-3 FITC

应用备注

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

说明

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

限制

仅限研究用
缓冲液

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C

储存方法

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Os, Bürgler, Ribes, Funderud, Wang, Thompson, Tjønnfjord, Bogen, Munthe: "Chronic lymphocytic leukemia cells are activated and proliferate in response to specific T helper cells." in: Cell reports, Vol. 4, Issue 3, pp. 566-77, (2013) (PubMed).

Dai, Jayapal, Tay, Reghunathan, Lin, Too, Lim, Chan, Kemeny, Floto, Smith, Melendez, MacAry: "Differential signal transduction, membrane trafficking, and immune effector functions mediated by FcgammaRI versus FcgammaRIIa." in: Blood, Vol. 114, Issue 2, pp. 318-27, (2009) (PubMed).

Dutertre, Bonnin-Gélizé, Pulford, Bourel, Fridman, Teillaud: "A novel subset of NK cells expressing high levels of inhibitory FcgammaRIIB modulating antibody-dependent function." in: Journal of leukocyte biology, Vol. 84, Issue 6, pp. 1511-20, (2008) (PubMed).

Boruchov, Heller, Veri, Bonvini, Ravetch, Young: "Activating and inhibitory IgG Fc receptors on human DCs mediate opposing functions." in: The Journal of clinical investigation, Vol. 115, Issue 10, pp. 2914-23, (2005) (PubMed).

Vely, Gruel, Moncuit, Cochet, Rouard, Dare, Galon, Sautes, Fridman, Teillaud: "A new set of monoclonal antibodies against human Fc gamma RII (CD32) and Fc gamma RIII (CD16): characterization and use in various assays." in: Hybridoma, Vol. 16, Issue 6, pp. 519-28, (1998) (PubMed).
抗原

Fc gamma RII (CD32)

别名

CD32 (CD32 产品)

别名

CD32 antibody, CD32B antibody, FCG2 antibody, FCGR2 antibody, IGFR2 antibody, FCGR2B antibody, AI528646 antibody, F630109E10Rik antibody, Fc[g]RII antibody, FcgRII antibody, Fcgr2 antibody, Fcgr2a antibody, Fcr-2 antibody, Fcr-3 antibody, Ly-17 antibody, Ly-m20 antibody, LyM-1 antibody, Lym-1 antibody, FCRII antibody, FcRII antibody, Fc fragment of IgG receptor IIb antibody, Fc receptor, IgG, low affinity IIb antibody, FCGR2B antibody, Fcgr2b antibody

背景

Fc fragment of IgG receptor IIa,CD32 (FcgammaRII) is a low affinity receptor for aggregated IgG. It is strongly expressed on monocytes, granulocytes, myeloid and myeloblastic cell lines, and weakly on B cells, CD34+ bone marrow cells, and resting and activated platelets. After binding its ligand, CD32 induces IgG-mediated phagocytosis and oxidative burst in monocytes and neutrophils, whereas in B cells it mediates a negative signal. This polymorphic transmembrane glycoprotein is expressed not only in the activating (CD32a) and inhibitory isoform (CD32b), but also in individual variants with differing avidities for IgG subtypes (e.g. the CD32a131R and CD32a131H allotypes).,FCG2, FCGR2A, IGFR2

基因ID

2212

UniProt

P12318