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CD235a/GYPA 抗体 (PE)

Name: CD235a/GYPA 抗体 (PE)
Brand: antibodies-online
SKU: ABIN2749119
Availability: OutOfStock

GYPA 适用: 人, 大鼠 FACS 宿主: 小鼠 Monoclonal JC159 PE

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD235a (JC159) PE antibody (10 μL reagent / 100 μL of peripheral whole blood). (CD235a/GYPA 抗体 (PE))

FACS

Flow cytometry analysis (surface staining) of CD235a in human peripheral blood (erythrocytes and leukocytes) with anti-CD235a (JC159) PE. (CD235a/GYPA 抗体 (PE))

FACS

Separation of human erythrocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD235a (JC159) PE antibody (10 μL reagent / 100 μL of peripheral whole blood). (CD235a/GYPA 抗体 (PE))

FACS

3 images

(4 references)

产品编号 ABIN2749119

发货至: 中国

Datasheet as PDF

抗原 See all CD235a/GYPA (GYPA) 抗体

CD235a/GYPA (GYPA) (Glycophorin A (GYPA))
适用
131

17

12

8
人, 大鼠
宿主
74

63

10

1
小鼠
Compatible Secondaries
克隆类型
85

61
单克隆
标记
57

17

11

9

5

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1
This CD235a/GYPA antibody is conjugated to PE
应用范围
98

54

42

33

24

23

15

13

13

13

12

5

5

5

3

2

1

1

1
Flow Cytometry (FACS)

特异性

The mouse monoclonal antibody JC159 recognizes an epitope between amino acids 27 and 40 of the extracellular portion of CD235a (glycophorin A), a sialoglycoprotein expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL. The antibody does not react with glycophorin B.

交叉反应（详细）

Human, Rat

纯化方法

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

免疫原

Membrane preparation from splenic hairy cell leukemia

克隆位点

JC159

亚型

IgG1

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GYPA 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Glycophorin A (GYPA) antibody
GYPA 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal GYPA-280 unconjugated

anti-Glycophorin A (GYPA) (Internal Region) antibody
GYPA 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Glycophorin A (GYPA) antibody (FITC)
GYPA 适用: 人, 大鼠 FACS 宿主: 小鼠 Monoclonal JC159 FITC

anti-Glycophorin A (GYPA) antibody
GYPA 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal rGYPA-280 unconjugated Recombinant Antibody

anti-Glycophorin A (GYPA) antibody
GYPA 适用: 人, 大鼠 FACS, IHC (p), IHC (fro) 宿主: 小鼠 Monoclonal JC159 unconjugated

anti-Glycophorin A (GYPA) (C-Term) antibody
Verified GYPA 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated

anti-Glycophorin A (GYPA) (C-Term) antibody
GYPA 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Glycophorin A (GYPA) antibody
GYPA 适用: 人 IHC, StM 宿主: 兔 Monoclonal GYPA-3219R unconjugated Recombinant Antibody

应用备注

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

说明

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

限制

仅限研究用
缓冲液

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C

储存方法

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Yamauchi, Takenaka, Urata, Shima, Kikushige, Tokuyama, Iwamoto, Nishihara, Iwasaki, Miyamoto, Honma, Nakao, Matozaki, Akashi: "Polymorphic Sirpa is the genetic determinant for NOD-based mouse lines to achieve efficient human cell engraftment." in: Blood, Vol. 121, Issue 8, pp. 1316-25, (2013) (PubMed).

Beck, Jagodzinski, Eller, Thelian, Matyas, Kunz, Alving: "Platelets and erythrocyte-bound platelets bind infectious HIV-1 in plasma of chronically infected patients." in: PLoS ONE, Vol. 8, Issue 11, pp. e81002, (2013) (PubMed).

Alijotas-Reig, Palacio-Garcia, Llurba, Vilardell-Tarres: "Cell-derived microparticles and vascular pregnancy complications: a systematic and comprehensive review." in: Fertility and sterility, Vol. 99, Issue 2, pp. 441-9, (2013) (PubMed).

Maijenburg, Kleijer, Vermeul, Mul, van Alphen, van der Schoot, Voermans: "The composition of the mesenchymal stromal cell compartment in human bone marrow changes during development and aging." in: Haematologica, Vol. 97, Issue 2, pp. 179-83, (2012) (PubMed).
抗原

CD235a/GYPA (GYPA) (Glycophorin A (GYPA))

别名

CD235a (GYPA 产品)

别名

GYPA antibody, gpa antibody, AI853584 antibody, CD235a antibody, GPA antibody, GPErik antibody, GPSAT antibody, HGpMiV antibody, HGpMiXI antibody, HGpSta(C) antibody, MN antibody, MNS antibody, PAS-2 antibody, glycophorin A (MNS blood group) antibody, glycophorin A antibody, GYPA antibody, Gypa antibody

背景

Glycophorin A (MNS blood group),CD235a (glycophorin A, GPA) is a transmembrane sialoglycoprotein expressed on erythrocytes and their precursors. Similarly to CD235b (glycophorin B, GPB), these molecules provide the cells with a large mucin-like surface, which minimalizes aggregation between erythrocytes in the circulation. GPA is the carrier of blood group M and N specificities, while GPB accounts for S, s and U specificities. CD235a is a receptor of Hsa, a Streptococcus adhesin.,Glycophorin A, GYPA, GPA, PAS-2, Sialoglycoprotein alpha, MN sialoglycoprotein, GPSAT, GPErik, MSN blood group

基因ID

2993

UniProt

P02724

途径

Maintenance of Protein Location