PCLO 抗体
Quick Overview for PCLO 抗体 (ABIN2749094)
抗原
See all PCLO 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-PCLO Purified
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特异性
- The mouse monoclonal antibody PCLO-01 recognizes PCLO (Piccolo), a more than 400 kDa multidomain protein expressed mainly in the presynaptic cytoplasmatic matrix of the neurons.
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交叉反应 （详细）
- Human
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纯化方法
- Purified by protein-A affinity chromatography.
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纯度
- > 95 % (by SDS-PAGE)
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免疫原
- Human recombinant PCLO protein
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亚型
- IgG1
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anti-Piccolo (Presynaptic Cytomatrix Protein) (PCLO) antibody (PE)
PCLO 适用: 人 ICFC 宿主: 小鼠 Monoclonal PCLO-01 PE
anti-Piccolo (Presynaptic Cytomatrix Protein) (PCLO) (AA 1-356) antibodyPCLO 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Piccolo (Presynaptic Cytomatrix Protein) (PCLO) antibodyPCLO 适用: 人 WB, IHC, FM 宿主: 兔 Polyclonal unconjugated
anti-Piccolo (Presynaptic Cytomatrix Protein) (PCLO) (C-Term) antibodyPCLO 适用: 人, 小鼠, 大鼠, Cow, 豚鼠, 马, Pig, 兔, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
anti-Piccolo (Presynaptic Cytomatrix Protein) (PCLO) antibodyPCLO 适用: 人 WB, FM, ELISA, IF 宿主: 小鼠 Monoclonal 6H9-B6 unconjugated
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应用备注
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Flow cytometry: Recommended dilution: 1-5 μg/mL, intracellular staining.
Western blotting: A small splice variant of PCLO can be analyzed by Western blotting, however, the whole protein is too large (over 400 kDa) to be successfully processed by SDS PAAGE. -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Do not freeze.
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- PCLO (Piccolo (Presynaptic Cytomatrix Protein) (PCLO))
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别名
- PCLO
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背景
- Piccolo presynaptic cytomatrix protein,PCLO (piccolo, also known as aczonin) is a large (more than 400 kDa) multidomain protein of the presynaptic cytomatrix in neurons, that is present in all vertebrate synapses, but is absent from invertebrates. It contains zinc finger and coiled-coil sequences, as well as N-terminal glutamine-rich sequence, and C-terminal PDZ domain followed by two C2 domains (C2A and C2B). In vitro binding and transfection experiments suggested that PCLO binds to multiple proteins including profilin and L-type calcium channels. It is involved in neurotransmitter release and insulin secretion.,ACZ, PCH3, piccolo, aczonin
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基因ID
- 27445
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UniProt
- Q9Y6V0
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途径
- Hormone Transport, Synaptic Vesicle Exocytosis
抗原
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