HLAG 抗体
(10 references)-
- 抗原 See all HLAG 抗体
- HLAG (HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This HLAG antibody is un-conjugated
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应用范围
- ELISA, Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- The antibody 87G recognizes both membrane-bound and soluble forms of HLA-G (HLA-G1 and HLA-G5). HLA-G belongs to the MHC Class I molecules (MHC Class Ib, nonclassical) and it is expressed on the surface of trophoblast cells.
- 无交叉反应
- 小鼠, 大鼠
- 交叉反应 （详细）
- Human
- 纯化方法
- Purified by protein-A affinity chromatography.
- 纯度
- > 95 % (by SDS-PAGE)
- 免疫原
- HLA-B27 transgenic mice were imunized with H-2 identical murine cells transfected with and expressing genes encoding HLA-G and human beta2-microglobulin.
- 克隆位点
- 87G
- 亚型
- IgG2a
- Top Product
- Discover our top product HLAG Primary Antibody
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anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody
HLAG 适用: 人 WB, IHC (fro), IHC (p) 宿主: 小鼠 Monoclonal MEM-G-1 unconjugated
anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody (PE)HLAG 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-G-9 PE
anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibodyHLAG 适用: 人 ELISA, FACS, IHC (fro), IP, ICC 宿主: 小鼠 Monoclonal MEM-G-9 unconjugated
anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibodyHLAG 适用: 人 ELISA, FACS, IHC (fro), IP, ICC 宿主: 小鼠 Monoclonal MEM-G-9 unconjugated
anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody (FITC)HLAG 适用: 人 FACS 宿主: 小鼠 Monoclonal 87G FITC
anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody (FITC)HLAG 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-G-9 FITC
anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody (Biotin)HLAG 适用: 人 ELISA, FACS, IHC (fro), IP, ICC 宿主: 小鼠 Monoclonal MEM-G-9 Biotin
anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody (APC)HLAG 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-G-9 APC
anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody (PE)HLAG 适用: 人 FACS 宿主: 小鼠 Monoclonal 87G PE
anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) (AA 62-89) antibodyHLAG 适用: 人 WB, ELISA, FACS, IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Flow cytometry: Extracellular and intracellular staining, Recommended dilution: 2 μg/mL, positive control: JEG-3 human choriocarcinoma epithelial cell line.
Immunohistochemistry (frozen sections): Recommended dilution: 10 μg/mL, incubation: 20 min at 25 °C, positive tissue: extravillous cytotrophoblast.
ELISA: Recommended dilution: 1 μg/mL, positive control: JEG-3 human choriocarcinoma epithelial cell line. The antibody 87G has been tested as the capture antibody in a sandwich ELISA for analysis of human HLA-G in combination with antibody W6/32. - 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store at 2-8°C. Do not freeze.
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: "Immune regulation by pretenders: cell-to-cell transfers of HLA-G make effector T cells act as regulatory cells." in: Blood, Vol. 109, Issue 5, pp. 2040-8, (2007) (PubMed).
: "The surface expression of HLA-F on decidual trophoblasts increases from mid to term gestation." in: Journal of reproductive immunology, Vol. 72, Issue 1-2, pp. 18-32, (2006) (PubMed).
: "HLA-G proteins in cancer: do they provide tumor cells with an escape mechanism?" in: Cancer research, Vol. 65, Issue 22, pp. 10139-44, (2005) (PubMed).
: "HLA-G antigen and parturition: maternal serum, fetal serum and amniotic fluid levels during pregnancy." in: Fetal diagnosis and therapy, Vol. 19, Issue 5, pp. 404-9, (2004) (PubMed).
: "Necrotic feature of the trophoblasts lacking HLA-G expression in normal and pre-eclamptic placentas." in: American journal of reproductive immunology (New York, N.Y. : 1989), Vol. 49, Issue 3, pp. 174-82, (2003) (PubMed).
: "Protein expression and peptide binding suggest unique and interacting functional roles for HLA-E, F, and G in maternal-placental immune recognition." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 3, pp. 1376-84, (2003) (PubMed).
: "Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules." in: Human immunology, Vol. 64, Issue 3, pp. 315-26, (2003) (PubMed).
: "Binding analysis of HLA-G specific antibodies to hematopoietic cells isolated from leukemia patients." in: Neoplasma, Vol. 50, Issue 5, pp. 331-8, (2003) (PubMed).
: "Analysis of HLA-G expression in malignant hematopoetic cells from leukemia patients." in: Leukemia research, Vol. 27, Issue 7, pp. 643-8, (2003) (PubMed).
: "HLA-G1 co-expression boosts the HLA class I-mediated NK lysis inhibition." in: International immunology, Vol. 13, Issue 2, pp. 193-201, (2001) (PubMed).
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: "Immune regulation by pretenders: cell-to-cell transfers of HLA-G make effector T cells act as regulatory cells." in: Blood, Vol. 109, Issue 5, pp. 2040-8, (2007) (PubMed).
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- 抗原
- HLAG (HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG))
- 别名
- HLA-G (HLAG 产品)
- 别名
- MHC-G antibody, B-F antibody, B-F-S04 antibody, B-F-S05 antibody, B-F-S06 antibody, B-F-S07 antibody, B-FI antibody, B-FIV antibody, BF2 antibody, BFa2 antibody, BFw-03 antibody, BFw-05 antibody, BFz-01 antibody, major histocompatibility complex, class I, G antibody, MHC BF1 class I antibody, HLA-G antibody, BF1 antibody
- 背景
- Major histocompatibility complex, class I, G,Human leukocyte antigen G (HLA-G), belonging to MHC class I glycoproteins, plays important roles in both physiological and pathological immunotolerance. It gives an inhibitory signal to cytotoxic T cells, NK cells, monocytes, and some other immune cells. It also induces regulatory T cells and anti-inflammatory macrophages. HLA-G is important e.g. for maternal tolerance to the fetus, and for immunomodulation in particular adult tissues, such as in cornea, pancreatic islets, thymus and other. On the other hand, it is expressed in many solid and hematologic malignancies, where it contributes to evasion of the immune surveillance. HLA-G expression pattern in cancer is an important prognostic factor regarding a poor clinical outcome. Unlike most other MHC glycoproteins, HLA-G acts as an immune checkpoint molecule rather than as an antigen presenting molecule. It concerns both transmembrane and soluble HLA-G isoforms. Among other, HLA-G can promote Th2 immunological response and downregulate Th1 immunological response. For its benefits regarding allograft tolerance, including embryo implantation, soluble HLA-G (sHLA-G) can be used as a marker of developmental potential of embryos during the process of in vitro fertilization. Similarly, sHLA-G concentrations in maternal serum are decreased in preeclampsia. Transplanted patients with increased sHLA-G serum levels have improved allograft acceptance. On the other hand, increased sHLA-G can also indicate presence of malignant (sometimes also of benign) tumor cells. Another important topic is induction of HLA-G expression (sometimes associated with shedding of HLA-G from the cell surface) by some anti-cancer or anti-viral therapies, which can weaken the therapy effect. Monitoring of HLA-G in patients thus has a wide usage.
- 基因ID
- 3135
- UniProt
- P17693
- 途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints, Human Leukocyte Antigen (HLA) in Adaptive Immune Response
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