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CD4 抗体 (PE)

Name: CD4 抗体 (PE)
Brand: antibodies-online
SKU: ABIN2749065
Availability: OutOfStock

CD4 适用: 大鼠 FACS 宿主: 小鼠 Monoclonal OX-35 PE

Flow cytometry surface staining pattern of rat splenocyte suspension stained using anti-rat CD4 (OX-35) PE antibody (concentration in sample 5 μg/mL). (CD4 抗体 (PE))

FACS

Separation of rat CD4 positive cells (red-filled) from rat CD4 negative cells (black-dashed) in flow cytometry analysis (surface staining) of rat splenocyte suspension stained using anti-rat CD4 (OX-35) PE antibody (concentration in sample 5 μg/mL). (CD4 抗体 (PE))

FACS

2 images

(10 references)

产品编号 ABIN2749065

发货至: 中国

Datasheet as PDF

抗原 See all CD4 抗体

CD4
适用
331

200

108

46

24

20

19

18

14

10

10

10

10

8

7

6

6

6

6

5

2

2

1

1

1

1
大鼠
宿主
319

144

106

3

3

1
小鼠
Compatible Secondaries
克隆类型
433

138

3
单克隆
标记
185

67

57

46

34

14

12

10

10

9

9

8

5

5

4

4

4

4

4

4

4

4

4

4

4

4

4

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1
This CD4 antibody is conjugated to PE
应用范围
454

189

130

89

88

66

55

42

39

34

29

25

10

7

7

7

6

6

5

3

3

3

2

2

2

1

1

1

1

1

1
Flow Cytometry (FACS)

特异性

The mouse monoclonal antibody OX-35 reacts with an extracellular epitope of rat CD4 transmembrane glycoprotein (55 kDa).

交叉反应（详细）

Rat

纯化方法

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

免疫原

MLR generated rat Th cells

克隆位点

OX-35

亚型

IgG2a kappa

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CD4 适用: 小鼠 FACS, IP, IHC (fro), ICC, Func 宿主: 大鼠 Monoclonal GK1-5 unconjugated

anti-CD4 (CD4) (N-Term) antibody (FITC)
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anti-CD4 (CD4) (N-Term) antibody (PE)
CD4 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-241 PE

应用备注

Flow cytometry: Recommended dilution: 1-5 μg/mL.

说明

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.

限制

仅限研究用
浓度

0.5 mg/mL

缓冲液

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C

储存方法

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Viel, Lemarié, Benkirane, Paradis, Schiffrin: "Immune regulation and vascular inflammation in genetic hypertension." in: American journal of physiology. Heart and circulatory physiology, Vol. 298, Issue 3, pp. H938-44, (2010) (PubMed).

Monzon-Casanova, Steiniger, Schweigle, Clemen, Zdzieblo, Starick, Müller, Wang, Rhost, Cardell, Pyz, Herrmann: "CD1d expression in paneth cells and rat exocrine pancreas revealed by novel monoclonal antibodies which differentially affect NKT cell activation." in: PLoS ONE, Vol. 5, Issue 9, (2010) (PubMed).

Baba, Iwasaki, Maruoka, Suzuki, Tomaru, Ikeda, Yoshiki, Kasahara, Ishizu: "Rat CD4+CD8+ macrophages kill tumor cells through an NKG2D- and granzyme/perforin-dependent mechanism." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 5, pp. 2999-3006, (2008) (PubMed).

Ramiro-Puig, Pérez-Cano, Ramos-Romero, Pérez-Berezo, Castellote, Permanyer, Franch, Izquierdo-Pulido, Castell: "Intestinal immune system of young rats influenced by cocoa-enriched diet." in: The Journal of nutritional biochemistry, Vol. 19, Issue 8, pp. 555-65, (2008) (PubMed).

Baba, Ishizu, Iwasaki, Suzuki, Tomaru, Ikeda, Yoshiki, Kasahara: "CD4+/CD8+ macrophages infiltrating at inflammatory sites: a population of monocytes/macrophages with a cytotoxic phenotype." in: Blood, Vol. 107, Issue 5, pp. 2004-12, (2006) (PubMed).

Gelderman, Hultqvist, Holmberg, Olofsson, Holmdahl: "T cell surface redox levels determine T cell reactivity and arthritis susceptibility." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 34, pp. 12831-6, (2006) (PubMed).

Yan, Devos, Yu, Xia, Rutgeerts, Goebels, Segers, Lin, Vandeputte, Waer: "Pathogenesis of autoimmunity after xenogeneic thymus transplantation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 12, pp. 5936-46, (2003) (PubMed).

Salomon, Netzer, Wildbaum, Schif-Zuck, Maor, Karin: "Targeting the function of IFN-gamma-inducible protein 10 suppresses ongoing adjuvant arthritis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 169, Issue 5, pp. 2685-93, (2002) (PubMed).

Tsuji, Kawaguchi, Wada, Nagoya, Inobe, Yamashita, Ishii, Uede: "Adenovirus-mediated in vivo B7-1 gene transfer induces anti-tumor immunity against pre-established primary tumor and pulmonary metastasis of rat osteosarcoma." in: Cancer gene therapy, Vol. 9, Issue 9, pp. 747-55, (2002) (PubMed).

Dragun, Lukitsch, Tullius, Qun, Park, Schneider, Luft, Haller: "Inhibition of intercellular adhesion molecule-1 with antisense deoxynucleotides prolongs renal isograft survival in the rat." in: Kidney international, Vol. 54, Issue 6, pp. 2113-22, (1999) (PubMed).
抗原

CD4

别名

CD4 (CD4 产品)

别名

cd4 antibody, L3T4 antibody, Ly-4 antibody, CD4mut antibody, W3/25 antibody, p55 antibody, CD4 molecule antibody, carcinoembryonic antigen-related cell adhesion molecule 5 antibody, T-cell surface glycoprotein CD4 antibody, CD4 antigen antibody, Cd4 molecule antibody, CD4 antibody, LOC100125525 antibody, cd4 antibody, Cd4 antibody

背景

CD4 Molecule,CD4 (T4) is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1), HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1), IL-16 (binds to CD4 domain 3), human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin. Intracellular ligands: p56LckCD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus, CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).,T4/Leu-3, L3T4

基因ID

24932

UniProt

P05540

途径

TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events