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CD247 抗体 (Tyr72)

Name: CD247 抗体 (Tyr72)
Brand: antibodies-online
SKU: ABIN2749060
Availability: OutOfStock

CD247 适用: 人, 小鼠 WB, FACS, ICC 宿主: 小鼠 Monoclonal EM-26 unconjugated

Anti-Hu CD3 zeta (pY72) Purified (clone EM-26) specificity verification by WB. (CD247 抗体 (Tyr72))

Reactivity of the monoclonal antibodies EM-26 (anti-CD3 zeta phospho-Tyr72) and EM-17 (anti-CD3 zeta phospho-Tyr153) with phosphorylated particular human CD3 zeta mutants. (CD247 抗体 (Tyr72))

Detection of phosphorylated CD3 zeta (; light blue) in immunological synapse formed between the lymph node naïve T cells from AND TCR transgenic mice and DCEK cells loaded with MCC peptide, after 20 min. (CD247 抗体 (Tyr72))

Immunocytochemistry detection of phosphorylated CD3 zeta (EM-26, light blue) in immunological synapse formed between the lymph node naive T cells from AND TCR transgenic mice and DCEK cells loaded with MCC peptide, after 20 min. (CD247 抗体 (Tyr72))

ICC

Anti-Hu CD3 zeta (p72) purified antibody (clone EM-26) works in Flow Cytometry application Analysis of the antibody staining was performed on Jurkat cells treated or untreated with pervanadate (PV) prior to the fixation and permeabilization of cell suspension with cold methanol. (CD247 抗体 (Tyr72))

FACS

Separation of pervanadate stimulated Jurkat cell suspension stained using anti-human CD3 zeta (pY72) (EM-26) purified antibody (concentration in sample 3 μg/mL, GAM APC, red-filled) from pervanadate stimulated Jurkat cell suspension unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining). (CD247 抗体 (Tyr72))

FACS

6 images

(1 reference)

产品编号 ABIN2749060

发货至: 中国

Datasheet as PDF

抗原 See all CD247 抗体

CD247 (CD247 Molecule (CD247))
抗原表位
13

13

8

7

7

6

5

5

4

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Tyr72
适用
88

38

16

4

1

1

1

1

1
人, 小鼠
宿主
72

13

5

1
小鼠
Compatible Secondaries
克隆类型
71

20
单克隆
标记
50

8

8

5

4

4

2

2

2

2

2

2
This CD247 antibody is un-conjugated
应用范围
69

50

29

14

9

9

5

5

3

2

1

1

1
Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC)

特异性

The mouse monoclonal antibody EM-26 recognizes phosphorylated intracellular tyrosine 72 of CD3 zeta chain (CD247), which is a component of TCR/CD3 complex expressed on T cells.

交叉反应（详细）

Human, Mouse

纯化方法

Purified by protein-A affinity chromatography.

纯度

> 95 % (by SDS-PAGE)

内毒素水平

Low Endotoxin

免疫原

A phospho specific peptide corresponding to the amino acids surrounding tyrosine 72 of mouse CD3 zeta linked to KLH

克隆位点

EM-26

亚型

IgG2b
anti-CD247 Molecule (CD247) (AA 1-164) antibody
CD247 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated

anti-CD247 Molecule (CD247) (AA 1-164) antibody
CD247 适用: 人 WB, ELISA, IP, IHC (p) 宿主: 小鼠 Monoclonal 4A12-F6 unconjugated

anti-CD247 Molecule (CD247) (AA 88-153) antibody
CD247 适用: 人 WB, ELISA, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-CD247 Molecule (CD247) (AA 50-164) antibody
CD247 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-CD247 Molecule (CD247) antibody
CD247 适用: 人 WB, ELISA, FACS, IHC, ICC 宿主: 小鼠 Monoclonal 4B10 unconjugated

anti-CD247 Molecule (CD247) antibody
CD247 适用: 人 WB, FACS, IHC 宿主: 兔 Monoclonal unconjugated

anti-CD247 Molecule (CD247) (AA 1-165) antibody
CD247 适用: 人 WB, IP, IHC (p), EIA 宿主: 小鼠 Monoclonal 4A12-F6 unconjugated

anti-CD247 Molecule (CD247) (AA 51-100) antibody
CD247 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-CD247 Molecule (CD247) (C-Term) antibody
CD247 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-CD247 Molecule (CD247) (pTyr142) antibody
CD247 适用: 人, 小鼠 WB, FACS 宿主: 小鼠 Monoclonal EM-54 unconjugated

应用备注

Western blotting: Recommended dilution: 2 - 5 μg/mL, positive control: Jurkat cells lysate treated with pervanadate, splenocyte lysate of Balb/c or F1 mouse treated with pervanadate, non-reducing conditions recommended.
Flow cytometry: Intracellular staining, recommended dilution: 1-9 μg/mL, positive control: Jurkat cells treated with pervanadate, T-cells from lymph nodes of OT-1 mouse treated with pervanadate.

限制

仅限研究用
浓度

1 mg/mL

缓冲液

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C

储存方法

Store at 2-8°C. Do not freeze.
Dopfer, Schöpf, Louis-Dit-Sully, Dengler, Höhne, Klescová, Prouza, Suchanek, Reth, Schamel: "Analysis of novel phospho-ITAM specific antibodies in a S2 reconstitution system for TCR-CD3 signalling." in: Immunology letters, Vol. 130, Issue 1-2, pp. 43-50, (2010) (PubMed).
抗原

CD247 (CD247 Molecule (CD247))

别名

CD3 zeta (CD247 产品)

别名

CD3-ZETA antibody, CD3H antibody, CD3Q antibody, CD3Z antibody, T3Z antibody, TCRZ antibody, 4930549J05Rik antibody, A430104F18Rik antibody, AW552088 antibody, Cd3 antibody, Cd3-eta antibody, Cd3-zeta antibody, Cd3h antibody, Cd3z antibody, Cd3zeta antibody, T3z antibody, Tcrk antibody, Tcrz antibody, LOC100136514 antibody, LOC100136549 antibody, LOC100305023 antibody, CD247 antibody, TCRzeta antibody, CD247 molecule antibody, CD247 antigen antibody, CD3zeta-2 antibody, CD3zeta-1 antibody, Cd247 molecule antibody, CD247 antibody, Cd247 antibody, cd3z antibody, cd247 antibody

背景

CD247 Molecule,CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta (CD247). These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.,CD3 zeta chain, CD247, T3Z, TCRz, IMD25

基因ID

919

UniProt

P20963

途径

TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway