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L-Selectin 抗体

Name: L-Selectin 抗体
Brand: antibodies-online
SKU: ABIN2749031
Availability: OutOfStock

SELL 适用: 人 FACS, WB, IP, IHC (fro), Func 宿主: 小鼠 Monoclonal DREG56 unconjugated

Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD62L (DREG56) purified antibody (low endotoxin, concentration in sample 1 μg/mL) GAM APC. (L-Selectin 抗体)

FACS

Separation of human CD62L positive lymphocytes (red-filled) from CD62L negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD62L (DREG56) purified antibody (low endotoxin, concentration in sample 1 μg/mL) GAM APC. (L-Selectin 抗体)

FACS

2 images

(6 references)

产品编号 ABIN2749031

发货至: 中国

Datasheet as PDF

抗原 See all L-Selectin (SELL) 抗体

L-Selectin (SELL) (Selectin L (SELL))
适用
104

70

34

17

9

6

4

3

2

2

1
人
宿主
80

61

50

1
小鼠
Compatible Secondaries
克隆类型
138

49

3
单克隆
标记
69

28

22

16

13

7

4

4

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1
This L-Selectin antibody is un-conjugated
应用范围
142

60

50

44

33

19

16

11

10

9

5

5

4

3

2

2

2

1

1

1

1

1

1

1
Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

特异性

The mouse monoclonal antibody DREG56 recognizes an extracellular epitope of CD62L / L-selectin, a 65-76 kDa cell surface protein, expressed by neutrophils, monocytes, and subsets of T, B, and NK cells, that interacts with specific carbohydrates exposed on activated endothelial cells.

交叉反应（详细）

Human

纯化方法

Purified by protein-A affinity chromatography.

纯度

> 95 % (by SDS-PAGE)

内毒素水平

Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

免疫原

PMA-activated human peripheral blood leukocytes

克隆位点

DREG56

亚型

IgG1

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应用备注

Functional application: Particularly effective in blocking of lymphocyte CD62L-mediated binding to peripheral lymph node HEV.
Flow cytometry: Recommended dilution: 2 μg/mL.
Western blotting: Non-reducing conditions.

限制

仅限研究用
浓度

1 mg/mL

缓冲液

Phosphate buffered saline (PBS), pH 7.4

储存液

Azide free

储存条件

4 °C

储存方法

Store at 2-8°C. Do not freeze.
Tu, Mao, Zheng, Liu, Chiu, Qin, Chan, Lam, Guan, Zhang, Guan, Yuen, Peiris, Lau: "Cytotoxic T lymphocytes established by seasonal human influenza cross-react against 2009 pandemic H1N1 influenza virus." in: Journal of virology, Vol. 84, Issue 13, pp. 6527-35, (2010) (PubMed).

Killock, Parsons, Zarrouk, Ameer-Beg, Ridley, Haskard, Zvelebil, Ivetic: "In Vitro and in Vivo Characterization of Molecular Interactions between Calmodulin, Ezrin/Radixin/Moesin, and L-selectin." in: The Journal of biological chemistry, Vol. 284, Issue 13, pp. 8833-45, (2009) (PubMed).

Xu, Chen, Shang, Cui, Luo, Chen, Ba, Zeng: "Critical role of Lck in L-selectin signaling induced by sulfatides engagement." in: Journal of leukocyte biology, Vol. 84, Issue 4, pp. 1192-201, (2008) (PubMed).

Jutila, Kurk, Jackiw, Knibbs, Stoolman: "L-selectin serves as an E-selectin ligand on cultured human T lymphoblasts." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 169, Issue 4, pp. 1768-73, (2002) (PubMed).

Kishimoto, Warnock, Jutila, Butcher, Lane, Anderson, Smith: "Antibodies against human neutrophil LECAM-1 (LAM-1/Leu-8/DREG-56 antigen) and endothelial cell ELAM-1 inhibit a common CD18-independent adhesion pathway in vitro." in: Blood, Vol. 78, Issue 3, pp. 805-11, (1991) (PubMed).

Kishimoto, Jutila, Butcher: "Identification of a human peripheral lymph node homing receptor: a rapidly down-regulated adhesion molecule." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 6, pp. 2244-8, (1990) (PubMed).
抗原

L-Selectin (SELL) (Selectin L (SELL))

别名

CD62L (SELL 产品)

别名

CD62L antibody, LAM1 antibody, LECAM1 antibody, LEU8 antibody, LNHR antibody, LSEL antibody, LYAM1 antibody, PLNHR antibody, TQ1 antibody, A.11 antibody, L-selectin antibody, LECAM-1 antibody, SELE antibody, AI528707 antibody, Lnhr antibody, Ly-22 antibody, Ly-m22 antibody, Lyam-1 antibody, Lyam1 antibody, selectin L antibody, selectin, lymphocyte antibody, SELL antibody, Sell antibody

背景

Selectin L,CD62L (L-selectin) is an adhesion glycoprotein that is constitutively expressed on the cell surface of leukocytes and mediates their homing to inflammatory sites and peripheral lymph nodes by enabling rolling along the venular wall. CD62L is also involved in activation-induced neutrophil aggregation. Activation-dependent CD62L shedding, however, counteracts neutrophil rolling. CD62L has also signaling roles including enhance of chemokine receptor expression. Similarly to CD62P, the major ligand of CD62L is PSGL-1 (P-selectin glycoprotein ligand-1).,L-selectin, LSEL, SELL, LAM-1, LECAM1, LEU8, LNHR, LYAM1, PLNHR

基因ID

6402

UniProt

P14151