CD16 抗体
(6 references)-
- 抗原 See all CD16 抗体
- CD16
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适用
- 人, Non-Human Primate
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD16 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunoprecipitation (IP), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- The mouse monoclonal antibody 3G8 recognizes an extracellular epitope of CD16, a low affinity receptor for aggregated IgG (FcgammaRIII antigen). CD16 exists in two different isoforms: CD16a (FcgammaRIIIA, 50-65 kDa, expressed on NK-cells, monocytes and macrophages) and CD16b (FcgammaRIIIB, 48 kDa, mainly expressed on neutrophils). Regarding CD16-158V/F polymorphism, the antibody 3G8 detects both 158V and 158F allotype on natural NK cells.
- 交叉反应 （详细）
- Human, Non-Human Primates
- 纯化方法
- Purified by protein-A affinity chromatography.
- 纯度
- > 95 % (by SDS-PAGE)
- 内毒素水平
- Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
- 免疫原
- Human neutrophils
- 克隆位点
- 3G8
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product CD16 Primary Antibody
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anti-CD16 (CD16) antibody
CD16 适用: 人, Non-Human Primate FACS, IP, IHC (fro) 宿主: 小鼠 Monoclonal 3G8 unconjugated
anti-CD16 (CD16) antibody (FITC)CD16 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal LNK16 FITC
anti-CD16 (CD16) antibodyCD16 适用: 人 FACS, IP, WB 宿主: 小鼠 Monoclonal MEM-154 unconjugated
anti-CD16 (CD16) antibodyCD16 适用: 人, Pig, Non-Human Primate FACS 宿主: 小鼠 Monoclonal MEM-168 unconjugated
anti-CD16 (CD16) antibodyCD16 适用: 人 FACS, IP, WB, Func 宿主: 小鼠 Monoclonal MEM-154 unconjugated
anti-CD16 (CD16) antibody (Biotin)CD16 适用: 人 FACS, IP, WB 宿主: 小鼠 Monoclonal MEM-154 Biotin
anti-CD16 (CD16) (AA 17-208) antibodyCD16 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 2G10A9 unconjugated
anti-CD16 (CD16) antibody (PE-DyLight 594)CD16 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal 3G8 PE-DyLight 594
anti-CD16 (CD16) antibodyCD16 适用: 人 WB, IHC (p) 宿主: 小鼠 Monoclonal 2B1 unconjugated
anti-CD16 (CD16) antibodyCD16 适用: 人 FACS 宿主: 小鼠 Monoclonal GRM1 unconjugated
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- 应用备注
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Functional application: In vitro stimulation of NK cell proliferation, blocking of IgG binding and phagocytosis, inhibition of cytotoxic activity, in vivo NK cell depletion.
Flow cytometry: Recommended dilution: 6 μg/mL.
Immunohistochemistry (frozen sections): Acetone fixation. - 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- Phosphate buffered saline (PBS), pH 7.4
- 储存液
- Azide free
- 储存条件
- 4 °C
- 储存方法
- Store at 2-8°C. Do not freeze.
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: "The lytic potential of human liver NK cells is restricted by their limited expression of inhibitory killer Ig-like receptors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 183, Issue 3, pp. 1789-96, (2009) (PubMed).
: "Use of an anti-CD16 antibody for in vivo depletion of natural killer cells in rhesus macaques." in: Immunology, Vol. 124, Issue 2, pp. 215-22, (2008) (PubMed).
: "Fc gamma RIIIa expression is not increased on natural killer cells expressing the Fc gamma RIIIa-158V allotype." in: Cancer research, Vol. 68, Issue 4, pp. 976-80, (2008) (PubMed).
: "Identification of a human peripheral blood monocyte subset that differentiates into osteoclasts." in: Arthritis research & therapy, Vol. 8, Issue 5, pp. R152, (2007) (PubMed).
: "Down-regulation of activating Fcgamma receptors on monocytes of patients with rheumatoid arthritis upon methotrexate treatment." in: Rheumatology (Oxford, England), Vol. 44, Issue 6, pp. 729-34, (2005) (PubMed).
: "Expression of functional CD32 molecules on human NK cells is determined by an allelic polymorphism of the FcgammaRIIC gene." in: Blood, Vol. 91, Issue 7, pp. 2369-80, (1998) (PubMed).
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: "The lytic potential of human liver NK cells is restricted by their limited expression of inhibitory killer Ig-like receptors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 183, Issue 3, pp. 1789-96, (2009) (PubMed).
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- 抗原
- CD16
- 别名
- CD16 (CD16 产品)
- 别名
- Fcgr3 antibody, Fcgr3a antibody, CD16 antibody, FCGR3 antibody, FCGR3@ antibody, CD16b antibody, FCG3 antibody, FCR-10 antibody, FCRIII antibody, FCRIIIb antibody, FcRIII antibody, Fc fragment of IgG, low affinity IIa, receptor antibody, Fc fragment of IgG, low affinity IIIa, receptor (CD16a) antibody, Fc fragment of IgG receptor IIIb antibody, Fc fragment of IgG receptor IIIa antibody, Fc receptor, IgG, low affinity III antibody, Fc-gamma RIII antibody, Fcgr2a antibody, FCGR3A antibody, FCGR3B antibody, Fcgr3 antibody, FCGR3 antibody
- 背景
- CD16 (FcgammaRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcgammaRIII is expressed in two forms –, FcgammaRIII-A and -B. FcgammaRIII-A is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FcepsilonRI-gamma subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcgammaRIII-A is associated, moreover, with FcepsilonRI-beta subunit. Besides IgG, FcgammaRIII-A can be triggered also by oligomeric IgE. FcgammaRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.,FcgammaRIII, IGFR3, FCRIII
- 途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
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