CD16 抗体
(4 references)-
- 抗原 See all CD16 抗体
- CD16
-
适用
- 人
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- This CD16 antibody is un-conjugated
-
应用范围
- Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB), Functional Studies (Func)
- 特异性
- The antibody MEM-154 reacts with an extracellular epitope on CD16 antigen that is residing in proximity to FG loop (probably BC or C'E loop). CD16 is a low affinity receptor for aggregated IgG (FcgammaRIII antigen). The antibody MEM-154 reacts with CD16+ granulocytes, and it can be used for mapping CD16-158V/F polymorphism on NK cells, as it requires presence of V at amino acid 158.
- 交叉反应 （详细）
- Human
- 纯化方法
- Purified by protein-A affinity chromatography.
- 纯度
- > 95 % (by SDS-PAGE)
- 内毒素水平
- Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
- 免疫原
- Human granulocytes
- 克隆位点
- MEM-154
- 亚型
- IgG1
- Top Product
- Discover our top product CD16 Primary Antibody
-
anti-CD16 (CD16) antibody
CD16 适用: 人, Non-Human Primate FACS, IP, IHC (fro) 宿主: 小鼠 Monoclonal 3G8 unconjugated
anti-CD16 (CD16) antibody (FITC)CD16 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal LNK16 FITC
anti-CD16 (CD16) antibodyCD16 适用: 人 FACS, IP, WB 宿主: 小鼠 Monoclonal MEM-154 unconjugated
anti-CD16 (CD16) antibodyCD16 适用: 人, Pig, Non-Human Primate FACS 宿主: 小鼠 Monoclonal MEM-168 unconjugated
anti-CD16 (CD16) antibody (Biotin)CD16 适用: 人 FACS, IP, WB 宿主: 小鼠 Monoclonal MEM-154 Biotin
anti-CD16 (CD16) (AA 17-208) antibodyCD16 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 2G10A9 unconjugated
anti-CD16 (CD16) antibodyCD16 适用: 人, Non-Human Primate FACS, IP, Func, IHC (fro) 宿主: 小鼠 Monoclonal 3G8 unconjugated
anti-CD16 (CD16) antibody (PE-DyLight 594)CD16 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal 3G8 PE-DyLight 594
anti-CD16 (CD16) antibodyCD16 适用: 人 WB, IHC (p) 宿主: 小鼠 Monoclonal 2B1 unconjugated
anti-CD16 (CD16) antibodyCD16 适用: 人 FACS 宿主: 小鼠 Monoclonal GRM1 unconjugated
-
- 应用备注
-
Functional application: The antibody MEM-154 blocks binding of human IgG to FcγRIII.
Flow cytometry: Recommended dilution: 5-10 μg/mL, positive control: PBL (peripheral blood lymphocytes). The antibody MEM-154 does not react with CD16a present on NK cells in many subjects.
Western blotting: Non-reducing conditions. - 限制
- 仅限研究用
-
- 浓度
- 1 mg/mL
- 缓冲液
- Phosphate buffered saline (PBS), pH 7.4
- 储存液
- Azide free
- 储存条件
- 4 °C
- 储存方法
- Store at 2-8°C. Do not freeze.
-
-
: "An afucosylated anti-CD20 monoclonal antibody with greater antibody-dependent cellular cytotoxicity and B-cell depletion and lower complement-dependent cytotoxicity than rituximab." in: Molecular immunology, Vol. 50, Issue 3, pp. 134-41, (2012) (PubMed).
: "Fc gammaRIIIa-158V/F polymorphism influences the binding of IgG by natural killer cell Fc gammaRIIIa, independently of the Fc gammaRIIIa-48L/R/H phenotype." in: Blood, Vol. 90, Issue 3, pp. 1109-14, (1997) (PubMed).
: "A triallelic Fc gamma receptor type IIIA polymorphism influences the binding of human IgG by NK cell Fc gamma RIIIa." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 156, Issue 8, pp. 2948-55, (1996) (PubMed).
: "The binding epitopes of human CD16 (Fc gamma RIII) monoclonal antibodies. Implications for ligand binding." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 4, pp. 1576-81, (1996) (PubMed).
-
: "An afucosylated anti-CD20 monoclonal antibody with greater antibody-dependent cellular cytotoxicity and B-cell depletion and lower complement-dependent cytotoxicity than rituximab." in: Molecular immunology, Vol. 50, Issue 3, pp. 134-41, (2012) (PubMed).
-
- 抗原
- CD16
- 别名
- CD16 (CD16 产品)
- 别名
- Fcgr3 antibody, Fcgr3a antibody, CD16 antibody, FCGR3 antibody, FCGR3@ antibody, CD16b antibody, FCG3 antibody, FCR-10 antibody, FCRIII antibody, FCRIIIb antibody, FcRIII antibody, Fc fragment of IgG, low affinity IIa, receptor antibody, Fc fragment of IgG, low affinity IIIa, receptor (CD16a) antibody, Fc fragment of IgG receptor IIIb antibody, Fc fragment of IgG receptor IIIa antibody, Fc receptor, IgG, low affinity III antibody, Fc-gamma RIII antibody, Fcgr2a antibody, FCGR3A antibody, FCGR3B antibody, Fcgr3 antibody, FCGR3 antibody
- 背景
- CD16 (FcgammaRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcgammaRIII is expressed in two forms –, FcgammaRIII-A and -B. FcgammaRIII-A is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FcepsilonRI-gamma subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcgammaRIII-A is associated, moreover, with FcepsilonRI-beta subunit. Besides IgG, FcgammaRIII-A can be triggered also by oligomeric IgE. FcgammaRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.,FcgammaRIII, IGFR3, FCRIII
- 途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
-
