Western Blotting (WB), ELISA, Immunoprecipitation (IP)
交叉反应
大鼠, 小鼠, 猴
产品特性
The G0S2-specific antibodies were generated using synthetic peptide derived from the from the C-terminal region of the G0S2 protein from within the region spanning AA 79-103. The G0S2-specific antibodies were affinity purified over immobilized antigen based affinity chromatography, and the purified immunoglobulins are stabilized in antibody stabilization buffer for long-term storage.
纯化方法
Affinity Purified
免疫原
Synthetic peptide corresponding to unique amino acid sequences on G0S2 protein.
Antibody was tested in ELISA and western blotting applications at 1:500 dilution using ABIN1040787 samples. Antibody dilutions for this antibody is for reference only, investigators are expected to determine the optimalconditions. Application of this antibody in other protocols has not yet tested. WB: > 1:500 IMM & IP pull-down assays: n.d. IHC: n.d. Investigators using this antibody in protocols other than listed above can request acomplimentary sample of this antibody. This antibody detects a band of 13 kDa in ABIN1040787 samples.The antibody also reacts to a 13 kDa protein in fat cells and TNF alpha induced THP cells.
限制
仅限研究用
状态
Liquid
浓度
0.51-0.54 μg/μL
缓冲液
The antiserum is supplied in antibody stabilization buffer with 0.02 % sodium azide.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Storage of very dilute antibody solutions is not recommended.
Lipolysis is the biochemical pathway responsible for the catabolism of triacylglycerol (TAG) stored in cellular lipid droplets by essential TAG hydrolase named adipose triglyceride lipase (ATGL). The ATGL action generates non-esterified fatty acids, which are subsequently used as energy substrate, essential precursors for lipid and membrane synthesis and mediator of cell signaling molecules. ATGL activity is regulated by several enzymes and binding factors including ATGL activator patatin-like phospholipase domain containing protein 58 (alpha/beta hydrolase containing protein 5) and ATGL inhibitor G0/G1 switch gene 2 (G0S2). Other factors/enzymes include hormone sensitive lipase E, and mono glyceride lipase constitute the basic \\