Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)
特异性
Recognizes human, rat and pig LPP.
交叉反应
人, 猪, 大鼠
纯化方法
Antigen affinity purified.
组件
Contains Positive Control: Human skin fibroblast protein, supplied at 1mg/ml in SDS (Laemmli) sample buffer. Use 25μl (25μg) per lane for Western blotting.
免疫原
Recombinant human lipoma preferred partner (LPP) (aa 1-109).
Lipoma preferred partner (LPP) is encoded by the preferred fusion partner gene of the high mobility group protein HMGI-C. LPP is frequently affected by chromosomal translocations in a major group of soft tissue lipomas, as well as in a parosteal lipoma and in pulmonary chondroid hamartomas. The resulting fusion proteins comprise three DNA binding domains of HMGI-C fused to two or three C-terminal LIM domains of LPP and a reciprocal product, respectively. Expression of a HMGI-C/LPP fusion protein causes malignant transformation of fibroblasts. Similar to its relatives zyxin and TRIP (thyroid receptor interacting protein 6) / ZRP-1 (zyxin-related protein 1), LPP consists of a N-terminal proline-rich domain followed by three C-terminal LIM domains (double zinc finger structures that are involved in protein-protein interactions). Like the cytoskeletal protein zyxin, LPP localizes to focal adhesions and cell-cell adherens junctions. LPP harbors a nuclear export signal and displays transcriptional activation capacity.