Recognizes an epitope within the peptide sequence aa 13-27 of mouse and rat Bmf. Detects bands of ~25 kDa and ~30 kDa by Western blot, corresponding to endogenous Bmf and BmfL.
交叉反应
小鼠, 大鼠
无交叉反应
人
交叉反应 (详细)
Does not cross-react with human Bmf.
纯度
>95 % (SDS-PAGE)
内毒素水平
<0.1EU/μg purified protein (LAL test, Lonza).
免疫原
Recombinant full length mouse Bmf and synthetic peptides corresponding to aa 13-27 and aa 112-126 of mouse Bmf.
Bmf (Bcl-2-modifying factor) belongs to the Bcl-2 protein family. Bcl-2 family members form hetero- or homo-dimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. Bmf contains a single Bcl-2 homology domain 3 (BH3), and has been shown to interact with other Bcl-2 proteins and functions as an apoptotic activator. Two different start sites can give rise to two proteins of 25 or 30 kDa. It is found to be sequestered to myosin V motors by its association with dynein light chain 2 (DLC2), which may be important for sensing intracellular damage and triggering apoptosis.