APAF1 抗体 (N-Term)
(3 references)-
- 抗原 See all APAF1 抗体
- APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))
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抗原表位
- AA 1-97, N-Term
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适用
- 人, 小鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This APAF1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC)
- 特异性
- Recognizes the CARD domain of human and mouse Apaf-1.
- 交叉反应
- 人, 小鼠
- 无交叉反应
- 大鼠
- 交叉反应 （详细）
- Does not cross-react with rat Apaf-1.
- 纯度
- >95 % (SDS-PAGE)
- 内毒素水平
- <0.1EU/μg purified protein (LAL test, Lonza).
- 免疫原
- Recombinant mouse Apaf-1 (aa 1-97) containing the N-terminal CARD domain.
- 克隆位点
- 18H2
- 亚型
- IgG2a
- Top Product
- Discover our top product APAF1 Primary Antibody
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anti-Apoptotic Peptidase Activating Factor 1 (APAF1) (AA 1-250) antibody
APAF1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Apoptotic Peptidase Activating Factor 1 (APAF1) (AA 31-350) antibodyAPAF1 适用: 人 WB, ELISA, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Apoptotic Peptidase Activating Factor 1 (APAF1) (AA 1138-1237) antibodyAPAF1 适用: 人 ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 2H9A1 unconjugated
anti-Apoptotic Peptidase Activating Factor 1 (APAF1) (AA 13-80) antibodyAPAF1 适用: 人, 小鼠, 大鼠 ELISA, IHC (p), IF (cc), IF (p), FACS, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Apoptotic Peptidase Activating Factor 1 (APAF1) (AA 1201-1248) antibodyAPAF1 适用: 人, 大鼠, 斑马鱼 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Apoptotic Peptidase Activating Factor 1 (APAF1) (AA 104-415) antibodyAPAF1 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Apoptotic Peptidase Activating Factor 1 (APAF1) (Internal Region) antibodyAPAF1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Apoptotic Peptidase Activating Factor 1 (APAF1) (C-Term) antibodyAPAF1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Apoptotic Peptidase Activating Factor 1 (APAF1) (C-Term) antibodyAPAF1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Apoptotic Peptidase Activating Factor 1 (APAF1) (AA 1-1205) antibodyAPAF1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- In PBS containing 0.02 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C. - 有效期
- 12 months
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: "Optimization of Experimental Variables Influencing Apoptosome Biosensor in HEK293T Cells." in: Sensors (Basel, Switzerland), Vol. 20, Issue 6, (2020) (PubMed).
: "A new split-luciferase complementation assay identifies pentachlorophenol as an inhibitor of apoptosome formation." in: FEBS open bio, Vol. 9, Issue 7, pp. 1194-1203, (2020) (PubMed).
: "Apoptosome formation upon overexpression of native and truncated Apaf-1 in cell-free and cell-based systems." in: Archives of biochemistry and biophysics, Vol. 642, pp. 46-51, (2019) (PubMed).
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: "Optimization of Experimental Variables Influencing Apoptosome Biosensor in HEK293T Cells." in: Sensors (Basel, Switzerland), Vol. 20, Issue 6, (2020) (PubMed).
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- 抗原
- APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))
- 别名
- Apaf-1 (APAF1 产品)
- 别名
- APAF1 antibody, ARK antibody, Apaf-1 antibody, Apaf1 antibody, CG6829 antibody, D-Apaf-1 antibody, DARK antibody, Dapaf-1 antibody, Dapaf-1/HAC-1 antibody, Dark antibody, Dark/Apaf-I antibody, Dark/Dapaf-1/HAC1 antibody, Dark/Hac-1/dApaf-1 antibody, Dark/Hac-1/dApaf1 antibody, Dmel\\CG6829 antibody, Hac-1 antibody, Hac-1/Dark antibody, Hac1 antibody, T1 antibody, anon-53Fa antibody, apaf-1 antibody, apaf1 antibody, arc antibody, ark antibody, dAPAF-1 antibody, dApaf-1 antibody, dApaf-1/DARK/HAC-1 antibody, dApaf1 antibody, dArk antibody, dapaf antibody, dapaf-1 antibody, dapaf-1L antibody, dapaf-1S antibody, dark antibody, dark/dapaf-1/hac-1 antibody, dark/hac-1/dapaf-1 antibody, hac-1 antibody, hac1 antibody, l(2)SH0173 antibody, APAF-1 antibody, CED4 antibody, 6230400I06Rik antibody, Apaf1l antibody, fog antibody, mKIAA0413 antibody, zgc:136543 antibody, Death-associated APAF1-related killer antibody, apoptotic peptidase activating factor 1 L homeolog antibody, apoptotic peptidase activating factor 1 antibody, Dark antibody, apaf1.L antibody, apaf1 antibody, APAF1 antibody, Apaf1 antibody
- 背景
- Apaf-1 is a cytoplasmic protein that forms one of the central hubs in the apoptosis regulatory network. It contains (from the N terminal) a caspase recruitment domain (CARD), an ATPase domain (NB-ARC), few short helical domains and then several copies of the WD40 repeat domain. Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome, mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis.
- UniProt
- O88879
- 途径
- p53 Pathway, Apoptosis, Caspase Cascade in Apoptosis, Tube Formation, Positive Regulation of Endopeptidase Activity
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