Cytokeratin 8/18 抗体
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- 抗原 See all Cytokeratin 8/18 products
- Cytokeratin 8/18
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Cytokeratin 8/18 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Immunoprecipitation (IP)
- 特异性
- Human Cytokeratin 8+18
- 纯化方法
- Protein G purified
- 免疫原
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A keratin preparation from a human carcinoma (K8.8) and PMC-42 human breast carcinoma cells (DC10) were used as the immunogen for this Cytokeratin 8 + 18 antibody cocktail.
Type of Immunogen: Cells - 克隆位点
- K8-8-DC10
- 亚型
- IgG1 kappa
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- 应用备注
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Approved: Flo (0.5 - 1 μg/10 x106), IF (1 - 2 μg/mL), IHC, IHC-P (0.5 - 1 μg/mL), IP, WB (0.5 - 1 μg/mL)
Usage: Cytokeratins are a group of proteins expressed in pairs in keratinized and non-keratinized epithelial tissue. Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). The pair are normally seen in simple but not stratified epithelial cells. This antibody cocktail recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. Immunohistochemical staining with this antibody cocktail is indistinguishable from that obtained with monoclonal antibody 5D3. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, pH 7.4, 0.1 mg/mL BSA, 0.05 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store at 2°C to 8°C.
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- 抗原
- Cytokeratin 8/18
- 别名
- Cytokeratin 8+18 (Cytokeratin 8/18 产品)
- 背景
- This antibody detects: Cytokeratin 8+18
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