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Cytokeratin 8/18 抗体

The 小鼠 单克隆 anti-Cytokeratin 8/18 antibody is suitable to detect Cytokeratin 8/18 in samples from 人. It has been validated for WB, IF, FACS, IHC, IHC (p) 和 IP.
产品编号 ABIN2740299
发货至: 中国
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Cytokeratin 8/18 抗体 (ABIN2740299)

抗原

Cytokeratin 8/18

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This Cytokeratin 8/18 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

克隆位点

K8-8-DC10
  • 特异性

    Human Cytokeratin 8+18

    纯化方法

    Protein G purified

    免疫原

    A keratin preparation from a human carcinoma (K8.8) and PMC-42 human breast carcinoma cells (DC10) were used as the immunogen for this Cytokeratin 8 + 18 antibody cocktail.

    Type of Immunogen: Cells

    亚型

    IgG1 kappa
  • 应用备注

    Approved: Flo (0.5 - 1 μg/10 x106), IF (1 - 2 μg/mL), IHC, IHC-P (0.5 - 1 μg/mL), IP, WB (0.5 - 1 μg/mL)

    Usage: Cytokeratins are a group of proteins expressed in pairs in keratinized and non-keratinized epithelial tissue. Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). The pair are normally seen in simple but not stratified epithelial cells. This antibody cocktail recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. Immunohistochemical staining with this antibody cocktail is indistinguishable from that obtained with monoclonal antibody 5D3.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    PBS,  pH 7.4, 0.1 mg/mL BSA, 0.05 % sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    Store at 2°C to 8°C.
  • 抗原

    Cytokeratin 8/18

    别名

    Cytokeratin 8+18

    背景

    This antibody detects: Cytokeratin 8+18
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