NCBP1
适用: 小鼠
ELISA, IHC (p), IF (cc), IF (p), ICC, IHC (fro)
宿主: 兔
Polyclonal
unconjugated
应用备注
ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1,0 mg/mL
缓冲液
Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
DO NOT FREEZE!
储存条件
4 °C
储存方法
Store the antibody undiluted at 2-8 °C.
抗原
NCBP1
(Nuclear Cap Binding Protein Subunit 1, 80kDa (NCBP1))
CBP80 antibody, CG7035 antibody, Dmel\\CG7035 antibody, EG:84H4.3 antibody, ncbp antibody, sto1 antibody, cbp80 antibody, AU014645 antibody, AW538051 antibody, NCBP antibody, Sto1 antibody, ncbp1 antibody, ABA HYPERSENSITIVE 1 antibody, ATCBP80 antibody, CAP-BINDING PROTEIN 80 antibody, ENS antibody, ENSALADA antibody, NUCLEAR CAP-BINDING PROTEIN CBP80 antibody, T10F5.8 antibody, T10F5_8 antibody, cap binding protein 80 antibody, nuclear cap binding protein subunit 1 antibody, nuclear cap binding protein subunit 1 S homeolog antibody, nuclear cap binding protein subunit 1, 80kDa antibody, nuclear cap binding protein subunit 1 L homeolog antibody, ARM repeat superfamily protein antibody, Nuclear cap-binding protein subunit 1-A antibody, Nuclear cap-binding protein subunit 1 antibody, Cbp80 antibody, ncbp1 antibody, ncbp1.S antibody, NCBP1 antibody, Ncbp1 antibody, ncbp1.L antibody, ABH1 antibody, nbp1a antibody, ncbp-1 antibody
背景
In eukaryotes, the majority of mRNAs have an m(7)G cap, which is added cotranscriptionally and plays a critical role in many aspects of mRNA metabolism. The effect of the cap on translation is mediated by the initiation factor eIF-4F, whereas the effect on pre-mRNA splicing involves a nuclear complex(CBC). CBC consists of two cap binding proteins CBP20 and CBP80, which mediate the stimulatory functions of the cap in pre-mRNA splicing, 3' end formation and U snRNA export. The genes CBC1 and CBC2 encode CBP80 and CBP20, respectively. CBP80 comprises three domains, each containing a MIF4G domain. CBP20 has an RNAP fold and associates with the second and third domains of CBP80. CBP also plays a role in nonsense-mediated decay(NMD), which eliminates mRNAs, which prematurely terminate translation.CBP80-bound mRNA undergoes a Synonyms: CBP80, NCBP, Nuclear cap-binding protein subunit 1