ELISA: 1: 5000approx. 1: 10000. WB: 1: 500approx. 1: 1000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
CBP20 antibody, CG12357 antibody, Dmel\\CG12357 antibody, 20kDa antibody, 5930413E18Rik antibody, AA536802 antibody, AI843301 antibody, C79367 antibody, CHUNP6917 antibody, zgc:92592 antibody, CBC2 antibody, NIP1 antibody, ATCBP20 antibody, CAP-binding protein 20 antibody, MLN1.12 antibody, MLN1_12 antibody, Ncbp2 antibody, cap binding protein 20 antibody, nuclear cap binding protein subunit 2 antibody, nuclear cap binding protein subunit 2, 20kDa antibody, nuclear cap binding protein subunit 2 L homeolog antibody, nuclear cap-binding protein subunit 2 antibody, CAP-binding protein 20 antibody, Nuclear cap-binding protein subunit 2 antibody, Cbp20 antibody, Ncbp2 antibody, ncbp2 antibody, NCBP2 antibody, ncbp2.L antibody, CBP20 antibody, ncbp-2 antibody
背景
In eukaryotes, the majority of mRNAs have an m(7)G cap, which is added cotranscriptionally and plays a critical role in many aspects of mRNA metabolism. The effect of the cap on translation is mediated by the initiation factor eIF-4F, whereas the effect on pre-mRNA splicing involves a nuclear complex (CBC). CBC consists of two cap binding proteins, CBP20 and CBP80, which mediate the stimulatory functions of the cap in pre-mRNA splicing, 3' end formation and U snRNA export. The genes CBC1 and CBC2 encode CBP80 and CBP20, respectively. CBP80 comprises three domains, each containing a MIF4G domain. CBP20 has an RNAP fold and associates with the second and third domains of CBP80. CBP also plays a role in nonsense-mediated decay (NMD), a process which eliminates mRNAs, and prematurely terminates translation. CBP80-bound mRNA undergoes a pioneer round of translation before CBP80-CBP20 are replaced by eIF4E, and Upf2 and Upf3 proteins.Synonyms: CBP20, Nuclear cap-binding protein subunit 2, PIG55