(4 references)-
- 抗原 See all SSEA-4 products
- SSEA-4 (Thiosulfate Sulfurtransferase 4 (SSEA-4))
- 适用
- 人, 小鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This SSEA-4 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Western Blotting (WB), BioImaging (BI)
- 品牌
- BD Pharmingen™
- 产品特性
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The MC813-70 monoclonal antibody reacts with Stage-Specific Embryonic Antigen-4 (SSEA-4), a carbohydrate epitope on the major ganglioside, but not the neutral glycolipid, of human teratocarcinoma cells. As its name implies, the expression of SSEA-4 is stage-specific and can be used to characterize embryonic cells and monitor their differentiation. However, its expression pattern differs in the human and mouse. In the human, SSEA-4 is found on teratocarcinoma (embryonal carcinoma or EC), embryonic inner cell mass (ICM), embryonic stem (ES) cells, and the K562 erythromyeloid leukeumia cell line. As human stem cells undergo differentiation, SSEA-4 expression is lost. In the mouse, SSEA-4 is found on oocytes and early cleavage-stage embryos, and primitive ectoderm, but not on EC, ICM, or ES cells. In some cases, SSEA-4 expression appears upon differentiation of mouse EC or ES cells. Immunofluorescent staining of human EC and ES cell lines. The 2102Ep cell line (clone 2/A6, Josephson et al, 2007) was obtained from Dr. Peter W. Andrews, the University of Sheffield, Sheffield, UK, and the H9 cell line (Thomson et al, 1998) was obtained from WiCell, Madison, WI. The cells were cultured, fixed, and stained with Purified Mouse anti-SSEA-4 monoclonal antibody (pseudo-colored green) according to the Recommended Assay Procedure. The second-step reagent was Alexa Fluor® 647 goat anti-mouse Ig (Invitrogen) and counter-staining was with Hoechst 33342 (pseudo-colored blue). The images were captured on a BD Pathway™ 855 Cell Analyzer and merged using BD Attovision™ software. LEFT PANEL: 2102Ep cells, 20X. MIDDLE PANEL: H9 cells, 20X. RIGHT PANEL: H9 cells, 4X.
BD Pharmingen™ Purified Mouse anti-SSEA-4 - Purified - Clone MC813-70 - Isotype Mouse IgG3, κ - Reactivity Hu, Ms - 0.1 mg - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Human Teratocarcinoma Cell Line
- 克隆位点
- MC813
- 亚型
- IgG3 kappa
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C.
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: "Qualification of embryonal carcinoma 2102Ep as a reference for human embryonic stem cell research." in: Stem cells (Dayton, Ohio), Vol. 25, Issue 2, pp. 437-46, (2007) (PubMed).
: "Heat shock 70-kDa protein 8 isoform 1 is expressed on the surface of human embryonic stem cells and downregulated upon differentiation." in: Stem cells (Dayton, Ohio), Vol. 23, Issue 10, pp. 1502-13, (2005) (PubMed).
: "Surface antigens of human embryonic stem cells: changes upon differentiation in culture." in: Journal of anatomy, Vol. 200, Issue Pt 3, pp. 249-58, (2002) (PubMed).
: "Embryonic stem cell lines derived from human blastocysts." in: Science (New York, N.Y.), Vol. 282, Issue 5391, pp. 1145-7, (1998) (PubMed).
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: "Qualification of embryonal carcinoma 2102Ep as a reference for human embryonic stem cell research." in: Stem cells (Dayton, Ohio), Vol. 25, Issue 2, pp. 437-46, (2007) (PubMed).
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- 抗原
- SSEA-4 (Thiosulfate Sulfurtransferase 4 (SSEA-4))
- 别名
- SSEA-4 (SSEA-4 产品)
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