R-Phycoerythrin 抗体 (Biotin)
This anti-R-Phycoerythrin antibody is a 小鼠 单克隆 antibody detecting R-Phycoerythrin in FACS. Suitable for .
Quick Overview for R-Phycoerythrin 抗体 (Biotin) (ABIN2689889)
抗原
R-Phycoerythrin
(Phycoerythrin, R-)
宿主
小鼠
克隆类型
单克隆
标记
This R-Phycoerythrin antibody is conjugated to Biotin
应用范围
Flow Cytometry (FACS)
克隆位点
E31
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品牌
- BD Pharmingen™
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产品特性
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The E31-1459 antibody reacts with R-Phycoerythrin (PE), a commonly used fluorochrome for flow cytometry, alone or as a tandem conjugate with other fluorochromes (eg, PE-Cy™7). The binding of the E31-1459 antibody to PE does not quench the fluorescence of the PE molecule. The use of biotinylated E31-1459 in a three-step staining procedure with Streptavidin-PE and a PE-conjugated primary antibody can specifically amplify the PE fluorescence. Amplification of PE-conjugated anti-mouse CD19 staining of BALB/c mouse splenocytes. Mouse splenocytes were incubated with PE-conjugated anti-mouse CD19 mAb 1D3 (Cat. No. 557399/553786) alone or followed by biotinylated mAb E31- 1459 and PE-conjugated Streptavidin (Cat. No. 554061, right panel). The overlayed histograms correspond to stained splenocytes (shaded histogram), unstained splenocytes (left panel, unshaded histogram), or splenocytes stained with biotinylated mAb E31-1459 and PE-conjugated Streptavidin (right panel, unshaded histogram). The signal-to-noise ratio of median fluorescence intensity (MFI) of the CD19+ population to the CD19- population in both panels (shaded histograms) is 87 for Panel A and 172 for right panel, demonstrating an approximate two-fold specific amplification of staining. Data shown is for gated lymphocytes based on light scatter. Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.
BD Pharmingen™ Biotin Mouse Anti-R-Phycoerythrin - Biotin - Clone E31-1459 - Isotype Mouse IgG1, κ - 0.1 mg -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- R-phycoerythrin
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亚型
- IgG1 kappa
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anti-Phycoerythrin, R- antibody (Alkaline Phosphatase (AP))
WB, ELISA, IHC 宿主: 山羊 Polyclonal Alkaline Phosphatase (AP)
anti-Phycoerythrin, R- antibody (Texas Red (TR))WB, DB, FM 宿主: 山羊 Polyclonal Texas Red (TR)
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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注意事项
- The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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- R-Phycoerythrin (Phycoerythrin, R-)
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别名
- R-Phycoerythrin
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物质类
- Chemical
抗原
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