(10 references)-
- 抗原 See all IL12RB2 抗体
- IL12RB2 (Interleukin 12 Receptor, beta 2 (IL12RB2))
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适用
- 小鼠
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宿主
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Armenian Hamster
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克隆类型
- 单克隆
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标记
- This IL12RB2 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS)
- 品牌
- BD Pharmingen™
- 产品特性
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The HAM10B9 antibody reacts with the β2subunit (IL-12Rβ2), of the mouse IL-12 receptor complex. The IL-12Rβ2 subunit associates with a β1 subunit to form a heterodimeric IL-12 receptor complex. Each one of the IL-12R subunits exhibits low affinity for IL-12, but in combination, they bind IL-12 with high affinity. The IL-12Rβ1 subunit interacts primarily with IL-12 p40 whereas the IL-12R β2 binds both to IL-12 p40 and IL-12 p35. IL-12Rβ1 is required for high affinity binding of IL-12 but IL-12R β2 is required for signaling. The cytoplasmic regions of the β1 and β2 subunits contain the box 1 and box 2 motifs found in other cytokine receptors such as gp130, LIFR and G-CSFR. Naive T cells do not express IL-12R but both IL-12R subunits can be induced on T cells by antigenic stimulation. The IL12R is also expressed on activated NK cells. Th1 cells express both IL-12R subunits while Th2 cells lose the β2 subunit during differentiation. The HAM10B9 antibody was generated by immunizing hamsters with mouse IL-12Rβ2 transfectants. Expression of cell surface IL-12R 2 by T helper cells. Mouse Th1 cell line, 2D6 (left panel) and Th2 cell line, D10 (center panel) were stained with purified anti-mouse IL-12 receptor ß2antibody (clone HAM10B9, 0.5 μg/test) followed by PE-conjugated anti-hamster IgG (0.25 μg, Cat . No. 554056). Staining with the HAM10B9 antibody (filled histograms) is compared to staining obtained using the isotype control antibody (open histograms). The histograms in the figure were derived from gated events with the forward and side light scatter characteristics of viable lymphocytes. Mouse splenocytes from C57BL/6 mice (right panel) were treated with an ammonium chloride lysing buffer to remove the red blood cells. Cells were subsequently cultured with ConA (2 μg/mL), PMA (50 ng/mL), Dextran sulfate (10 μg/mL), LPS (5 μg/mL), recombinant mouse IL-2 (10 ng/mL), recombinant mouse IL-12p70 (20 ng/mL) and anti-IL-4 antibody, clone 11B11 (5 μg/mL) for 5 days. Following culture the cells were harvested, washed, blocked with mouse Fc Block™ (Cat. No. 553141) and stained with purified anti-mouse IL-12 receptor β2 antibody (clone HAM10B9, 0.5 μg/test) followed by PE-conjugated anti-hamster IgG (0.25 μg, Cat . No. 554056) and Viaprobe (Cat. No. 555816). Staining with anti-mouse IL-12 receptor ß2antibody (clone HAM10B9, filled histograms) is compared to staining obtained using the isotype control antibody (Cat. No. 553969, open histograms). The histograms in the figure were derived from viable gated events (e.g. ViaProbe negative lymphocytes).
BD Pharmingen™ Purified Hamster Anti-Mouse IL-12 Receptor β2 - Purified - Clone HAM10B9 - Isotype Armenian Hamster IgG1, κ - Reactivity Ms - 0.1 mg - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Mouse IL-12Rbeta2 transfectants
- 克隆位点
- HAM10B9
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product IL12RB2 Primary Antibody
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anti-Interleukin 12 Receptor, beta 2 (IL12RB2) (AA 301-400) antibody
IL12RB2 适用: 人 WB, ELISA, IF (p), IHC (fro), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 12 Receptor, beta 2 (IL12RB2) (AA 1-862) antibodyIL12RB2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 12 Receptor, beta 2 (IL12RB2) (AA 105-214) antibodyIL12RB2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2H6 unconjugated
anti-Interleukin 12 Receptor, beta 2 (IL12RB2) (AA 756-783) antibodyIL12RB2 适用: 人 WB, IHC, FACS, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 12 Receptor, beta 2 (IL12RB2) (AA 759-788), (C-Term) antibodyIL12RB2 适用: 人 WB, FACS, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 12 Receptor, beta 2 (IL12RB2) (AA 559-647) antibodyIL12RB2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 12 Receptor, beta 2 (IL12RB2) (AA 285-441) antibodyIL12RB2 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 12 Receptor, beta 2 (IL12RB2) (AA 366-470) antibodyIL12RB2 适用: 人 ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 12 Receptor, beta 2 (IL12RB2) (AA 1-862) antibodyIL12RB2 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Interleukin 12 Receptor, beta 2 (IL12RB2) (C-Term) antibodyIL12RB2 适用: 人, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C.
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: "Role of IFN-gamma in Th1 differentiation: IFN-gamma regulates IL-18R alpha expression by preventing the negative effects of IL-4 and by inducing/maintaining IL-12 receptor beta 2 expression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 168, Issue 12, pp. 6165-72, (2002) (PubMed).
: "Differentiation of murine NK cells into distinct subsets based on variable expression of the IL-12R beta 2 subunit." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 9, pp. 4985-93, (2000) (PubMed).
: "IL-12 receptor beta 2 (IL-12R beta 2)-deficient mice are defective in IL-12-mediated signaling despite the presence of high affinity IL-12 binding sites." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 11, pp. 6221-8, (2000) (PubMed).
: "Role of IL-12 in intrathymic negative selection." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 8, pp. 4349-59, (1999) (PubMed).
: "Characterization of mouse interleukin-12 p40 homodimer binding to the interleukin-12 receptor subunits." in: European journal of immunology, Vol. 29, Issue 6, pp. 2007-13, (1999) (PubMed).
: "The interleukin-12/interleukin-12-receptor system: role in normal and pathologic immune responses." in: Annual review of immunology, Vol. 16, pp. 495-521, (1998) (PubMed).
: "Analysis of the multiple interactions between IL-12 and the high affinity IL-12 receptor complex." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 5, pp. 2174-9, (1998) (PubMed).
: "A functional interleukin 12 receptor complex is composed of two beta-type cytokine receptor subunits." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 24, pp. 14002-7, (1997) (PubMed).
: "Characterization of IL-12 receptor beta1 chain (IL-12Rbeta1)-deficient mice: IL-12Rbeta1 is an essential component of the functional mouse IL-12 receptor." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 4, pp. 1658-65, (1997) (PubMed).
: "The alphas, betas, and kinases of cytokine receptor complexes." in: Cell, Vol. 74, Issue 4, pp. 587-90, (1993) (PubMed).
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: "Role of IFN-gamma in Th1 differentiation: IFN-gamma regulates IL-18R alpha expression by preventing the negative effects of IL-4 and by inducing/maintaining IL-12 receptor beta 2 expression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 168, Issue 12, pp. 6165-72, (2002) (PubMed).
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- 抗原
- IL12RB2 (Interleukin 12 Receptor, beta 2 (IL12RB2))
- 别名
- IL-12 Receptor Beta2 (IL12RB2 产品)
- 别名
- IL12RB2 antibody, A930027I18Rik antibody, IL-12RB2 antibody, Ifnm antibody, interleukin 12 receptor subunit beta 2 antibody, interleukin 12 receptor, beta 2 antibody, IL12RB2 antibody, Il12rb2 antibody
- 途径
- JAK/STAT Signaling
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