FOXP3 抗体
(3 references)-
- 抗原 See all FOXP3 抗体
- FOXP3 (Forkhead Box P3 (FOXP3))
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适用
- 人, Baboon, Cynomolgus, 猕猴
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This FOXP3 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Intracellular Staining (ICS)
- 品牌
- BD Pharmingen™
- 产品特性
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The 259D/C7 antibody reacts with the human FoxP3 transcription factor, a member of the forkhead or winged helix family of transcription factors. The expression of FoxP3, also known as Scurfin, IPEX and JM2, has been found to be associated with CD4+ regulatory T cells and represents a specific marker for these cells. Flow-cytometric analysis has shown that FoxP3 is expressed by the majority of CD4+CD25+high T cells in peripheral blood while less than half of CD4+CD25int cell population are FoxP3 positive. Approximately 5-10 % of peripheral CD4+ cells are CD4+CD25+ T regulatory cells. T regulatory cells are thought to play a critical role in the control of T cell mediated autoimmunity by suppressing the proliferation and cytokine production of other T cells. To support this hypothesis, it has been found that FOXP3 is mutated in scurfy (sf) mice. The 259D/C7 antibody reacts with all currently identified isoforms of human FoxP3 and is cross-reactive with Cynomolgus, Rhesus and Baboon. Flow cytometric analysis of Purified anti-Human FoxP3 on resting PBMC. Human PBMC were fixed and permeabilized using working solutions of FoxP3 Buffers A and C (Cat. No. 560098. see Recommended Assay Procedure), Intracellular staining with Purified Mouse anti-Human FoxP3 (clone 259D/C7, Cat. No. 560044) was followed by secondary staining with APC Rat anti-Mouse IgG1 (
BD Pharmingen™ Purified Mouse anti-Human FoxP3 - Purified - Clone 259D/C7 - Isotype Mouse IgG1 - Reactivity Hu, Bab, Cyno, Rhe - 0.1 mg - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- FoxP3 recombinant protein
- 克隆位点
- 259D-C7
- 亚型
- IgG1
- Top Product
- Discover our top product FOXP3 Primary Antibody
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anti-Forkhead Box P3 (FOXP3) (AA 190-412) antibody
FOXP3 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Forkhead Box P3 (FOXP3) (C-Term) antibodyVerified FOXP3 适用: 小鼠 FACS, ELISA, IHC, IF 宿主: 山羊 Polyclonal unconjugated
anti-Forkhead Box P3 (FOXP3) (AA 297-431) antibodyFOXP3 适用: 人 FACS, ELISA, IHC 宿主: 小鼠 Monoclonal 4F12A2 unconjugated
anti-Forkhead Box P3 (FOXP3) (AA 191-412) antibodyFOXP3 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Forkhead Box P3 (FOXP3) (C-Term) antibodyVerified FOXP3 适用: 人 WB, FACS, ELISA, IHC, IF, ICC 宿主: 山羊 Polyclonal unconjugated
anti-Forkhead Box P3 (FOXP3) (N-Term) antibodyFOXP3 适用: 人, 小鼠, 大鼠, Cow, Pig, 马, 兔, 犬, 绵羊, 豚鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Forkhead Box P3 (FOXP3) (AA 191-412) antibodyFOXP3 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C1 unconjugated
anti-Forkhead Box P3 (FOXP3) (AA 1-431) antibodyFOXP3 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Forkhead Box P3 (FOXP3) antibody (APC)FOXP3 适用: 人, 小鼠 FACS 宿主: 小鼠 Monoclonal 3G3 APC
anti-Forkhead Box P3 (FOXP3) (AA 101-431) antibodyFOXP3 适用: 人, 小鼠, 大鼠 IHC (p) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C.
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: "Analysis of FOXP3 protein expression in human CD4+CD25+ regulatory T cells at the single-cell level." in: European journal of immunology, Vol. 35, Issue 6, pp. 1681-91, (2005) (PubMed).
: "Disruption of a new forkhead/winged-helix protein, scurfin, results in the fatal lymphoproliferative disorder of the scurfy mouse." in: Nature genetics, Vol. 27, Issue 1, pp. 68-73, (2001) (PubMed).
: "X-linked neonatal diabetes mellitus, enteropathy and endocrinopathy syndrome is the human equivalent of mouse scurfy." in: Nature genetics, Vol. 27, Issue 1, pp. 18-20, (2001) (PubMed).
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: "Analysis of FOXP3 protein expression in human CD4+CD25+ regulatory T cells at the single-cell level." in: European journal of immunology, Vol. 35, Issue 6, pp. 1681-91, (2005) (PubMed).
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- 抗原
- FOXP3 (Forkhead Box P3 (FOXP3))
- 别名
- FoxP3 (FOXP3 产品)
- 背景
- Synonyms: Scurfin, IPEX, JM2
- 途径
- Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation
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