(3 references)-
- 抗原 See all Fetal Hemoglobin (HbF) products
- Fetal Hemoglobin (HbF)
- 适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Fetal Hemoglobin antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS)
- 品牌
- BD Pharmingen™
- 产品特性
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Reacts with fetal hemoglobin (HbF), a form of hemoglobin present during fetal development. HbF resembles adult hemoglobin (HbA) in possessing two alpha chains but differs in possessing two gamma instead of two beta chains. In normal adults, synthesis of HbF does persist at very low levels (<1 % of total Hb) and is restricted to a small population of erythrocytes called F cells. Hemoglobin F-expressing erythrocytes are normally seen during pregnancy. An increase in the expression of fetal hemoglobin in adult peripheral red blood cells is a common feature in the genetic disorders of hemoglobin, sickle-cell disease (SCD) and beta thalassemia. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Profile of erythrocytes obtained from cord blood analyzed by flow cytometry. Second step staining with Cat. No. 555988.
BD Pharmingen™ Purified Mouse Anti-Human Fetal Hemoglobin - Purified - Clone 2D12 - Isotype Mouse IgG1, κ - Reactivity Hu - 0.1 mg - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 克隆位点
- 2D12
- 亚型
- IgG1 kappa
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C.
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: "Detection of hemoglobin variants in erythrocytes by flow cytometry." in: Cytometry, Vol. 35, Issue 3, pp. 242-8, (1999) (PubMed).
: "Estimation of fetal hemoglobin levels in individual red cells via fluorescence image cytometry." in: Cytometry, Vol. 20, Issue 3, pp. 261-7, (1995) (PubMed).
: "Immunochemical estimation of haemoglobin types in red blood cells by FACS analysis." in: British journal of haematology, Vol. 87, Issue 1, pp. 125-32, (1994) (PubMed).
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: "Detection of hemoglobin variants in erythrocytes by flow cytometry." in: Cytometry, Vol. 35, Issue 3, pp. 242-8, (1999) (PubMed).
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- 抗原
- Fetal Hemoglobin (HbF)
- 别名
- Fetal Hemoglobin (HbF 产品)
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