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- 抗原 See all FAS 抗体
- FAS (TNF Receptor Superfamily, Member 6 (FAS))
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适用
- 小鼠
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宿主
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Armenian Hamster
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克隆类型
- 单克隆
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标记
- This FAS antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunoprecipitation (IP), Cytotoxicity Test (CyTox)
- 品牌
- BD Pharmingen™
- 产品特性
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Fas antigen, CD95, is a 45 kDa cell-surface protein which can mediate apoptosis. It belongs to the TNF (tumor necrosis factor)/NGF receptor family. Expression of Fas has been described in the thymus, liver, heart, lung and ovary. Fas plays an important role in the apoptotic process that takes place during development. Monoclonal antibodies recognizing Fas such as Jo2 have cytolytic activity on cells expressing Fas. The cell death stimulated by Fas antibodies is characteristic of apoptosis and suggests that the lethal effects are a result of interaction of antibody with a functional Fas antigen as opposed to complement-mediated lysis. The Jo2 antibody recognizes mouse Fas. The Jo2 antibody shows cytolytic activity against cell lines expressing mouse Fas by inducing apoptosis. Intraperitoneal injections of Jo2 mAb have been shown to kill mice and induce apoptotic hepatocyte death. Jo2 mAb immunoprecipitates mouse Fas as a 45 kDa band from W4 cells. W4 cells are WR19L mouse lymphoma cells transformed with mouse Fas. The difference between the observed MW of Fas and that deduced from its amino acid sequence (Mr 34,971) may be due to glycosylation. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Expression of Fas on mouse thymocytes analyzed by flow cytometry. Thymocytes from a C57BL/6 mouse were incubated with either Jo2, followed by a FITC-conjugated mouse anti-hamster second step (filled histogram, or with second step alone (open histogram).
BD Pharmingen™ Purified Hamster Anti-Mouse CD95 - Purified - Clone Jo2 - Isotype Armenian Hamster IgG2, λ2 - Reactivity Ms - 0.5 mg - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- WR19L mouse lymphoma cells transformed with recombinant mouse Fas
- 克隆位点
- Jo2
- 亚型
- IgG2 lambda
- Top Product
- Discover our top product FAS Primary Antibody
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C.
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Selective apoptosis of CD4+CD8+ thymocytes by the anti-Fas antibody." in: The Journal of experimental medicine, Vol. 181, Issue 2, pp. 485-91, (1995) (PubMed).
: "Fas and activation-induced Fas ligand mediate apoptosis of T cell hybridomas: inhibition of Fas ligand expression by retinoic acid and glucocorticoids." in: The Journal of experimental medicine, Vol. 181, Issue 5, pp. 1673-82, (1995) (PubMed).
: "Increased Fas antigen expression in murine retrovirus-induced immunodeficiency syndrome, MAIDS." in: European journal of immunology, Vol. 24, Issue 10, pp. 2446-51, (1994) (PubMed).
: "Fas and perforin pathways as major mechanisms of T cell-mediated cytotoxicity." in: Science (New York, N.Y.), Vol. 265, Issue 5171, pp. 528-30, (1994) (PubMed).
: "Lethal effect of the anti-Fas antibody in mice." in: Nature, Vol. 364, Issue 6440, pp. 806-9, (1993) (PubMed).
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Selective apoptosis of CD4+CD8+ thymocytes by the anti-Fas antibody." in: The Journal of experimental medicine, Vol. 181, Issue 2, pp. 485-91, (1995) (PubMed).
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- 抗原
- FAS (TNF Receptor Superfamily, Member 6 (FAS))
- 别名
- CD95 (FAS 产品)
- 别名
- ALPS1A antibody, APO-1 antibody, APT1 antibody, CD95 antibody, FAS1 antibody, FASTM antibody, TNFRSF6 antibody, AI196731 antibody, APO1 antibody, TNFR6 antibody, Tnfrsf6 antibody, lpr antibody, Fas cell surface death receptor antibody, Fas (TNF receptor superfamily member 6) antibody, FAS antibody, Fas antibody, fas antibody
- 背景
- Synonyms: Fas/APO-1
- 分子量
- 45 kDaTarget Molecular Weight: Aqueous buffered solution containing ≤0.09% sodium azide.
- 途径
- p53 Pathway, Apoptosis, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
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