CD90.2 / Thy-1.2 抗体
(13 references)-
- 抗原 See all CD90.2 / Thy-1.2 products
- CD90.2 / Thy-1.2
- 适用
- 小鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This CD90.2 / Thy-1.2 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Cytotoxicity Test (CyTox)
- 品牌
- BD Pharmingen™
- 产品特性
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The 53-2.1 antibody reacts with the CD90.2 (Thy-1.2) alloantigen on thymocytes, most peripheral T lymphocytes, some intraepithelial T lymphocytes (IEL, DEC), epithelial cells, fibroblasts, neurons, hematopoietic stem cells, but not B lymphocytes, of most mouse strains. mAb 53-2.1 has been reported not to cross-react with Thy-1.1 (e.g., AKR/J, PL), or with rat Thy-1. CD90 is a GPI-anchored membrane glycoprotein of the Ig superfamily which is involved in signal transduction. In addition, there is evidence that CD90 mediates adhesion of thymocytes to thymic stroma. mAb 53-2.1 has been reported to block the binding of anti-mouse CD90.2 clone 30-H12 (Cat. No. 553009) to immobilized thymocyte membranes. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.
BD Pharmingen™ Purified Rat Anti-Mouse CD90.2 - Purified - Clone 53-2.1 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.5 mg - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Mouse Thymus / Spleen
- 克隆位点
- 53
- 亚型
- IgG2a kappa
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anti-CD90.2 / Thy-1.2 antibody (PE)
适用: 小鼠 FACS 宿主: 大鼠 Monoclonal 30-H12 PE
anti-CD90.2 / Thy-1.2 antibody (PE)适用: 小鼠 FACS 宿主: 小鼠 Monoclonal 1aG4-C5 PE
anti-CD90.2 / Thy-1.2 antibody (violetFluor™ 450)适用: 小鼠 FACS 宿主: 大鼠 Monoclonal 30-H12 violetFluor™ 450
anti-CD90.2 / Thy-1.2 antibody (PE-Cy7)适用: 小鼠 FACS 宿主: 大鼠 Monoclonal 30-H12 PE-Cy7
anti-CD90.2 / Thy-1.2 antibody适用: 小鼠 FACS 宿主: 大鼠 Monoclonal 30-H12 unconjugated
anti-CD90.2 / Thy-1.2 antibody (Biotin)适用: 小鼠 FACS 宿主: 大鼠 Monoclonal 30-H12 Biotin
anti-CD90.2 / Thy-1.2 antibody (FITC)适用: 小鼠 FACS 宿主: 大鼠 Monoclonal 30-H12 FITC
anti-CD90.2 / Thy-1.2 antibody (APC)适用: 小鼠 FACS 宿主: 大鼠 Monoclonal 30-H12 APC
anti-CD90.2 / Thy-1.2 antibody (SPRD)适用: 小鼠 FACS 宿主: 大鼠 Monoclonal 30-H12 SPRD
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- 应用备注
- Flow Cytometry : Mouse BD Fc Block™ purified anti-mouse CD16/CD32 clone 2.4G2 may help to reduce non-specific binding to cells bearing Fcγ-receptors. For staining in the presence of Mouse BD Fc Block™, a second step antibody which does not recognize the 2.4G2 mAb (Rat IgG2b, κ) must be used, such as FITC anti-rat IgG2a, mAb RG7/1.30 . Caution: Sodium azide is a reversible inhibitor of oxidative metabolism, therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also effect the results of functional studies, we recommend the NA/LE™ (No Azide/Low Endotoxin) antibody format for in vitro and in vivo use. Page 1 of 2553000 Rev. 12
- 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C.
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: "T helper cells in murine germinal centers are antigen-specific emigrants that downregulate Thy-1." in: The Journal of experimental medicine, Vol. 184, Issue 3, pp. 1083-91, (1997) (PubMed).
: "The appearance of Thy-1- donor T cells in the peripheral circulation 3-6 weeks after bone marrow transplantation suggests an extrathymic origin." in: International immunology, Vol. 8, Issue 2, pp. 171-6, (1996) (PubMed).
: "Differential Thy-1 expression by splenic fibroblasts defines functionally distinct subsets." in: Cellular immunology, Vol. 173, Issue 2, pp. 198-206, (1996) (PubMed).
: "Developmental regulation of Thy 1.2 rate of synthesis in the mouse cerebellum." in: Journal of neuroscience research, Vol. 42, Issue 2, pp. 220-7, (1996) (PubMed).
: "Thy-1 triggers mouse thymocyte apoptosis through a bcl-2-resistant mechanism." in: The Journal of experimental medicine, Vol. 179, Issue 3, pp. 785-96, (1994) (PubMed).
: "Lymphocyte development from stem cells." in: Annual review of immunology, Vol. 10, pp. 759-83, (1992) (PubMed).
: "Extrathymic differentiation of intraepithelial lymphocytes: generation of a separate and unequal T-cell repertoire?" in: Immunology today, Vol. 12, Issue 12, pp. 436-8, (1992) (PubMed).
: "Thy-1 supports adhesion of mouse thymocytes to thymic epithelial cells through a Ca2(+)-independent mechanism." in: The Journal of experimental medicine, Vol. 173, Issue 2, pp. 515-8, (1991) (PubMed).
: "TCR gamma/delta+ dendritic epidermal T cells as constituents of skin-associated lymphoid tissue." in: The Journal of investigative dermatology, Vol. 94, Issue 6 Suppl, pp. 58S-63S, (1990) (PubMed).
: "Thy-1 functions as a signal transduction molecule in T lymphocytes and transfected B lymphocytes." in: Nature, Vol. 322, Issue 6075, pp. 181-4, (1986) (PubMed).
: "Neuronal cell Thy-1 glycoprotein: homology with immunoglobulin." in: Science (New York, N.Y.), Vol. 216, Issue 4547, pp. 696-703, (1982) (PubMed).
: "Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens." in: Immunological reviews, Vol. 47, pp. 63-90, (1980) (PubMed).
: "T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views." in: The Journal of experimental medicine, Vol. 152, Issue 2, pp. 280-95, (1980) (PubMed).
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: "T helper cells in murine germinal centers are antigen-specific emigrants that downregulate Thy-1." in: The Journal of experimental medicine, Vol. 184, Issue 3, pp. 1083-91, (1997) (PubMed).
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- 抗原
- CD90.2 / Thy-1.2
- 别名
- CD90.2 (CD90.2 / Thy-1.2 产品)
- 别名
- CD90 antibody, T25 antibody, Thy-1 antibody, Thy-1.2 antibody, Thy1.1 antibody, Thy1.2 antibody, thymus cell antigen 1, theta antibody, Thy1 antibody
- 背景
- Synonyms: Thy-1.2
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