-
- 抗原 See all CD5 抗体
- CD5
-
适用
- 小鼠
-
宿主
- 大鼠
-
克隆类型
- 单克隆
-
标记
- This CD5 antibody is conjugated to Biotin
-
应用范围
- Flow Cytometry (FACS)
- 品牌
- BD Pharmingen™
- 产品特性
-
The 53-7.3 clone has been reported to react with a monomorphic epitope of CD5, a member of the scavenger receptor cysteine-rich protein superfamily and the major ligand of CD72, found on thymocytes, T lymphocytes, thymic NK-T cells, and a subset of B lymphocytes, but not on NK cells or splenic NK-T cells. The level of surface CD5 expression is developmentally regulated in the thymus, starting with low levels on CD4-CD8- thymocytes and increasing as they mature to CD4+CD8+ then CD4+CD8- or CD4-CD8+ thymocytes. Relatively high levels are maintained on peripheral T lymphocytes. The level of CD5 antigen detected on T helper cells has been reported to be somewhat higher than that on T cytotoxic/suppressor and B cells. Few, if any, intestinal intraepithelial lymphocytes bearing the γδ T-cell receptor express CD5. Phenotypic, anatomical, functional, developmental, and pathogenic characteristics of peripheral CD5+ B cells suggest that they may represent a distinct lineage, known as B-1 cells. The frequency of these CD5+ B cells has been reported to show strain-dependent variation. An additional population of CD5+ B lymphocytes resides in the thymus, where it matures from intrathymic B-cell progenitors. It has been proposed that CD5 is a costimulatory molecule which mediates interactions of cells in the immune system and negatively regulates signal transduction mediated by the T-cell receptor and B-cell receptor. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.
BD Pharmingen™ Biotin Rat Anti-Mouse CD5 - Biotin - Clone 53-7.3 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.1 mg - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Mouse Thymus / Spleen
- 克隆位点
- 53
- 亚型
- IgG2a kappa
- Top Product
- Discover our top product CD5 Primary Antibody
-
-
- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
-
- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
-
-
Natural killer cell receptor signaling." in: Current opinion in immunology, Vol. 15, Issue 3, pp. 308-14, (2003) (PubMed).
: "CD5 expression is developmentally regulated by T cell receptor (TCR) signals and TCR avidity." in: The Journal of experimental medicine, Vol. 188, Issue 12, pp. 2301-11, (1999) (PubMed).
: "A role for CD5 in cognate interactions between T cells and B cells, and identification of a novel ligand for CD5." in: International immunology, Vol. 10, Issue 8, pp. 1185-96, (1998) (PubMed).
: "A fundamental difference in the capacity to induce proliferation of naive T cells between CD28 and other co-stimulatory molecules." in: European journal of immunology, Vol. 28, Issue 3, pp. 926-35, (1998) (PubMed).
: "Mouse CD1-specific NK1 T cells: development, specificity, and function." in: Annual review of immunology, Vol. 15, pp. 535-62, (1997) (PubMed).
: "CD5-mediated negative regulation of antigen receptor-induced growth signals in B-1 B cells." in: Science (New York, N.Y.), Vol. 274, Issue 5294, pp. 1906-9, (1997) (PubMed).
: "Surface molecules that drive T cell development in vitro in the absence of thymic epithelium and in the absence of lineage-specific signals." in: Immunity, Vol. 6, Issue 3, pp. 245-55, (1997) (PubMed).
: "Phenotypes and invariant alpha beta TCR expression of peripheral V alpha 14+ NK T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 5, pp. 2076-82, (1997) (PubMed).
: "Presence of B cell progenitors in the thymus." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 9, pp. 4193-9, (1997) (PubMed).
: "A role for CD5 in TCR-mediated signal transduction and thymocyte selection." in: Science (New York, N.Y.), Vol. 269, Issue 5223, pp. 535-7, (1995) (PubMed).
: "Origin of murine B cell lineages." in: Annual review of immunology, Vol. 11, pp. 501-38, (1993) (PubMed).
: "Ly-1 (CD5), a membrane glycoprotein of mouse T lymphocytes and a subset of B cells, is a natural ligand of the B cell surface protein Lyb-2 (CD72)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 6, pp. 1630-4, (1992) (PubMed).
: "Phenotypic complexity of intraepithelial lymphocytes of the small intestine." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 6, pp. 1746-51, (1991) (PubMed).
: "Normal, autoimmune, and malignant CD5+ B cells: the Ly-1 B lineage?" in: Annual review of immunology, Vol. 6, pp. 197-218, (1988) (PubMed).
: "The "Ly-1 B" cell subpopulation in normal immunodefective, and autoimmune mice." in: The Journal of experimental medicine, Vol. 157, Issue 1, pp. 202-18, (1983) (PubMed).
: "Fluorescence analysis and anatomic distribution of mouse T lymphocyte subsets defined by monoclonal antibodies to the antigens Thy-1, Lyt-1, Lyt-2, and T-200." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 127, Issue 6, pp. 2594-604, (1982) (PubMed).
: "Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens." in: Immunological reviews, Vol. 47, pp. 63-90, (1980) (PubMed).
: "T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views." in: The Journal of experimental medicine, Vol. 152, Issue 2, pp. 280-95, (1980) (PubMed).
: "
-
Natural killer cell receptor signaling." in: Current opinion in immunology, Vol. 15, Issue 3, pp. 308-14, (2003) (PubMed).
-
- 抗原
- CD5
- 别名
- CD5 (CD5 产品)
- 别名
- CD5 antibody, Ly-1 antibody, Ly-12 antibody, Ly-A antibody, Lyt-1 antibody, LEU1 antibody, T1 antibody, CD5 molecule antibody, CD5 antigen antibody, CD5 molecule like antibody, Cd5 molecule antibody, CD5 antibody, Cd5 antibody, CD5L antibody
- 背景
- Synonyms: Ly-1
-