(6 references)-
- 抗原 See all CD27 抗体
- CD27 (CD27 Molecule (CD27))
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适用
- 小鼠, 大鼠
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宿主
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Armenian Hamster
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克隆类型
- 单克隆
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标记
- This CD27 antibody is conjugated to Biotin
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应用范围
- Flow Cytometry (FACS)
- 品牌
- BD Pharmingen™
- 产品特性
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The LG.3A10 antibody reacts with CD27, a lymphocyte-restricted member of the Tumor Necrosis Factor Receptor family which binds to CD70. The CD27 Molecule is a 45- kDa transmembrane glycoprotein which is constitutively expressed by lymphocytes of the T lineage: virtually all thymocytes and over 90 % of peripheral T cells bearing both αβ and γδ T-cell receptors. CD27 cooperates with the pre-TCR in mediating thymocyte differentiation and expansion. In addition, one to ten percent of mature peripheral B cells express CD27, and CD27's role in the differentiation of human plasma cells has been studied. Mouse NK cells, freshly isolated and IL-2-activated, also express CD27. In the bone marrow, CD27 is found on a progenitor population which provides short-term hematopoietic reconstitution. Cells of the myeloid lineage do not express CD27. Cross-linked LG.3A10 mAb has been reported to amplify the proliferative response of purified T lymphocytes to suboptimal mitogenic stimulation1 and to enhance NK-cell proliferation and IFN-γ production. In contrast, non-cross-linked LG.3A10 mAb inhibits CD3-induced pre-T cell development by interfering with the receptor-ligand interaction. This hamster mAb to a mouse leukocyte antigen has been observed to cross-react with a similar population of rat leukocytes. Expression of CD27 on mouse splenocytes. C57BL/6 splenocytes were stained simultaneously with PE-conjugated anti-mouse CD3e mAb 500A2 (Cat. No. 553240) and biotinylated mAb LG.3A10 (right panel), followed by Avidin-FITC (Cat. No. 554057). Flow cytometry was performed on a BD FACScan™ flow cytometry system.
BD Pharmingen™ Biotin Hamster Anti-Mouse CD27 - Biotin - Clone LG.3A10 - Isotype Armenian Hamster IgG1, κ - Reactivity Ms, Rat - 0.1 mg - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Armenian hamster fibroblast line ARHO12 transfected with mouse Cd27 cDNA
- 克隆位点
- LG-3A10
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product CD27 Primary Antibody
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anti-CD27 Molecule (CD27) (AA 1-260) antibody
CD27 适用: 人 WB, ELISA, RNAi 宿主: 小鼠 Monoclonal 1E2-A3 unconjugated
anti-CD27 Molecule (CD27) antibodyCD27 适用: 人 FACS, ELISA, IHC, IF, StM, Coat 宿主: 小鼠 Monoclonal LPFS2-1611 unconjugated
anti-CD27 Molecule (CD27) antibodyCD27 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD27 Molecule (CD27) (AA 20-191) antibodyCD27 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD27 Molecule (CD27) (AA 20-144) antibodyCD27 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 3B6D3 unconjugated
anti-CD27 Molecule (CD27) (AA 196-225) antibodyCD27 适用: 人 FACS, WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-CD27 Molecule (CD27) (AA 229-312) antibodyCD27 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD27 Molecule (CD27) antibodyCD27 适用: 人 FACS, IHC, StM 宿主: 小鼠 Monoclonal rLPFS2-1611 unconjugated Recombinant Antibody
anti-CD27 Molecule (CD27) antibodyCD27 适用: 人 FACS 宿主: 小鼠 Monoclonal LT27 unconjugated
anti-CD27 Molecule (CD27) (AA 20-191) antibodyCD27 适用: 人 FACS, ELISA 宿主: 兔 Monoclonal DM57 unconjugated Recombinant Antibody
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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: "Plasma cell generation from B-lymphocytes via CD27/CD70 interaction." in: Leukemia & lymphoma, Vol. 35, Issue 3-4, pp. 219-25, (2000) (PubMed).
: "CD27-mediated activation of murine NK cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 4, pp. 1741-5, (2000) (PubMed).
: "Expression of CD27 on murine hematopoietic stem and progenitor cells." in: Immunity, Vol. 12, Issue 2, pp. 193-9, (2000) (PubMed).
: "CD27 cooperates with the pre-T cell receptor in the regulation of murine T cell development." in: The Journal of experimental medicine, Vol. 184, Issue 2, pp. 675-85, (1996) (PubMed).
: "Novel mAbs reveal potent co-stimulatory activity of murine CD27." in: International immunology, Vol. 7, Issue 4, pp. 551-7, (1995) (PubMed).
: "Cloning and expression of murine CD27: comparison with 4-1BB, another lymphocyte-specific member of the nerve growth factor receptor family." in: European journal of immunology, Vol. 23, Issue 4, pp. 943-50, (1993) (PubMed).
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: "Plasma cell generation from B-lymphocytes via CD27/CD70 interaction." in: Leukemia & lymphoma, Vol. 35, Issue 3-4, pp. 219-25, (2000) (PubMed).
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- 抗原
- CD27 (CD27 Molecule (CD27))
- 别名
- CD27 (CD27 产品)
- 途径
- Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
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