(13 references)-
- 抗原 See all 2B4 (CD244) 抗体
- 2B4 (CD244) (Natural Killer Cell Receptor 2B4 (CD244))
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适用
- 小鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This 2B4 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 品牌
- BD Pharmingen™
- 产品特性
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CD244 is a member of the CD2 subset of the immunoglobulin superfamily (CD2 IgSF). It is expressed on all natural killer (NK) cells, IL-2-activated NK (LAK) cells, committed progenitors of NK cells, and a subset of T lymphocytes which mediate non-MHC-restricted cytoxicity, including dendritic epidermal T cells. The 2B4 antibody reacts with CD244.2, the 2B4 alloantigen which is expressed on C57BL/6 and C58/J mice, but not in most strains tested (A/J, AKR, BALB/c, CBA/J, CBA/N, C3H/He, C57BR, DBA/1, DBA/2, NZB, SJL/J, 129). At least two isoforms of CD244.2 protein, products of alternative splicing of hnRNA, are expressed on IL-2-activated C57BL/6 NK cells. They differ only in their cytoplasmic domains, with 2B4L (150-aa cytoplasmic tail) having inhibitory activity and 2B4S (93-aa tail) being stimulatory. The extracellular domain of CD244 is a ligand for another CD2 IgSF member, CD48. 2B4 antibody activates lytic and secretory functions of IL-2-cultured NK cells and CD244.2+ T cells. Two-color analysis of CD244.2 expression on LAK cells. C57BL/6 LAK cells were simultaneously stained with PE-conjugated PK136 (anti-mouse NK-1.1, Cat. No. 557391/553165, both panels) and purified 2B4 (right panel) monoclonal antibodies, followed by FITC-conjugated anti-mouse IgG2b mAb R12-3 (Cat. No. 553395, both panels). Flow cytometry was performed on a BD FACScan™ System .
BD Pharmingen™ Purified Mouse Anti-Mouse CD244.2 - Purified - Clone 2B4 - Isotype Mouse IgG2b, κ - Reactivity Ms - 0.1 mg - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- rIL-2-propagated NK1.1+ cells from C57BL/6 mice
- 克隆位点
- 2B4
- 亚型
- IgG2b kappa
- Top Product
- Discover our top product CD244 Primary Antibody
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anti-Natural Killer Cell Receptor 2B4 (CD244) (AA 20-221) antibody
CD244 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Natural Killer Cell Receptor 2B4 (CD244) (N-Term) antibodyCD244 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Natural Killer Cell Receptor 2B4 (CD244) (AA 22-221) antibodyCD244 适用: 人 ELISA, FACS 宿主: 兔 Monoclonal DM69 unconjugated Recombinant Antibody
anti-Natural Killer Cell Receptor 2B4 (CD244) (AA 151-250) antibodyCD244 适用: 人, 大鼠 WB, ELISA, IF (cc), IF (p), ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Natural Killer Cell Receptor 2B4 (CD244) (pTyr271) antibodyCD244 适用: 人 WB, ELISA, FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Natural Killer Cell Receptor 2B4 (CD244) (AA 26-212) antibodyCD244 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Natural Killer Cell Receptor 2B4 (CD244) (AA 22-224) antibodyCD244 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Natural Killer Cell Receptor 2B4 (CD244) antibodyCD244 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Natural Killer Cell Receptor 2B4 (CD244) antibodyCD244 适用: 人 FACS, IP 宿主: 小鼠 Monoclonal 2B4-69 unconjugated
anti-Natural Killer Cell Receptor 2B4 (CD244) (AA 1-365) antibodyCD244 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C.
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: "Identification of committed NK cell progenitors in adult murine bone marrow." in: European journal of immunology, Vol. 31, Issue 6, pp. 1900-9, (2001) (PubMed).
: "Molecular cloning of transmembrane and soluble forms of a novel rat natural killer cell receptor related to 2B4." in: Immunogenetics, Vol. 51, Issue 4-5, pp. 306-13, (2000) (PubMed).
: "2B4, the natural killer and T cell immunoglobulin superfamily surface protein, is a ligand for CD48." in: The Journal of experimental medicine, Vol. 188, Issue 11, pp. 2083-90, (1999) (PubMed).
: "The natural killer gene complex genetic locus Chok encodes Ly-49D, a target recognition receptor that activates natural killing." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 11, pp. 6330-5, (1999) (PubMed).
: "Characterization of a surface membrane molecule expressed by natural killer cells in most inbred mouse strains: monoclonal antibody C9.1 identifies an allelic form of the 2B4 antigen." in: Immunology, Vol. 96, Issue 3, pp. 491-7, (1999) (PubMed).
: "Characterization of inhibitory and stimulatory forms of the murine natural killer cell receptor 2B4." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 7, pp. 3870-5, (1999) (PubMed).
: "Gene structure of the murine NK cell receptor 2B4: presence of two alternatively spliced isoforms with distinct cytoplasmic domains." in: European journal of immunology, Vol. 29, Issue 8, pp. 2392-9, (1999) (PubMed).
: "Identification of the 2B4 molecule as a counter-receptor for CD48." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 11, pp. 5809-12, (1998) (PubMed).
: "The structure and ligand interactions of CD2: implications for T-cell function." in: Immunology today, Vol. 17, Issue 4, pp. 177-87, (1997) (PubMed).
: "2B4, a new member of the immunoglobulin gene superfamily, is expressed on murine dendritic epidermal T cells and plays a functional role in their killing of skin tumors." in: The Journal of investigative dermatology, Vol. 105, Issue 4, pp. 592-6, (1995) (PubMed).
: "Activation of murine epidermal gamma delta T cells through surface 2B4." in: European journal of immunology, Vol. 25, Issue 4, pp. 1117-20, (1995) (PubMed).
: "Cloning and characterization of the 2B4 gene encoding a molecule associated with non-MHC-restricted killing mediated by activated natural killer cells and T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 10, pp. 5328-37, (1993) (PubMed).
: "Pan natural killer cell monoclonal antibodies and their relationship to the NK1.1 antigen." in: Hybridoma, Vol. 8, Issue 6, pp. 605-14, (1990) (PubMed).
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: "Identification of committed NK cell progenitors in adult murine bone marrow." in: European journal of immunology, Vol. 31, Issue 6, pp. 1900-9, (2001) (PubMed).
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- 抗原
- 2B4 (CD244) (Natural Killer Cell Receptor 2B4 (CD244))
- 别名
- CD244.2 (CD244 产品)
- 背景
- Synonyms: 2B4 B6 Alloantigen
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