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- 抗原 See all CD11b (ITGAM) 抗体
- CD11b (ITGAM) (Integrin alpha M (ITGAM))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD11b antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 品牌
- BD Pharmingen™
- 产品特性
- The ICRF44 monoclonal antibody reacts with CD11b, the 165- kDa adhesion glycoprotein that associates with the 95- kDa integrin β2 (CD18) to form the CD11b/CD18 complex, also known as Mac-1 or CR3. CD11b is expressed on activated lymphocytes, monocytes, granulocytes, and a subset of NK cells. CD11b functions in cell-cell and cell-substrate interactions and is a receptor for iC3b, CD54 (ICAM-1), CD102 (ICAM-2) and CD50 (ICAM-3). This antibody significantly inhibits polymorphonuclear leukocyte aggregation in response to fMLP.
- 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Human monocytes.
- 克隆位点
- ICRF44
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product ITGAM Primary Antibody
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- 应用备注
- Immunohistochemistry: The ICRF44 (44) antibody is recommended to test for immunohistochemical staining of acetone-fixed frozen sections. Tissue tested was human spleen and tonsil. The antibody stains activated lymphocytes, monocytes, granulocytes and a subset of NK cells. The isotype control recommended for use with this antibody is purified mouse IgG1. For optimal indirect immunohistochemical staining, the ICRF44 (44) antibody should be titrated (1:10 to 1:50 dilution) and visualized via a three-step staining procedure in combination with polyclonal, biotin conjugated anti-mouse Igs (multiple adsorbed) as the secondary antibody and Streptavidin-HRP together with the DAB detection system. The clone ICRF44 (44) is not recommended for formalin-fixed paraffin embedded sections.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 62.5 μg/mL
- 缓冲液
- Aqueous buffered solution containing BSA, goat serum, and ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C.
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Interaction of proteinase 3 with CD11b/CD18 (beta2 integrin) on the cell membrane of human neutrophils." in: Journal of leukocyte biology, Vol. 74, Issue 4, pp. 551-7, (2003) (PubMed).
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Interaction of proteinase 3 with CD11b/CD18 (beta2 integrin) on the cell membrane of human neutrophils." in: Journal of leukocyte biology, Vol. 74, Issue 4, pp. 551-7, (2003) (PubMed).
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- 抗原
- CD11b (ITGAM) (Integrin alpha M (ITGAM))
- 别名
- CD11b/Mac-1 (ITGAM 产品)
- 别名
- CD11b/CD18 antibody, CR3 antibody, CR3A antibody, Cd11b antibody, F730045J24Rik antibody, Ly-40 antibody, MAC1 antibody, Mac-1 antibody, Mac-1a antibody, CD11B antibody, MAC-1 antibody, MAC1A antibody, MO1A antibody, SLEB6 antibody, C3bi antibody, integrin subunit alpha M antibody, integrin alpha M antibody, integrin, alpha M (complement component 3 receptor 3 subunit) antibody, integrin alpha-M antibody, ITGAM antibody, Itgam antibody, LOC100351865 antibody
- 背景
- Synonyms: Mac-1α, integrin αM subunit, CR3 α chain
- 途径
- Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation
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