Reacts with CD104, integrin β4 chain, a 205 kDa transmembrane glycoprotein, which associates with CD49f (integrin α6 chain) to form the α6/β4 (CD49f/CD104) complex. It is expressed on epithelial cells, Schwann cells, and some tumor cells. The CD49f/CD104 complex is located in the hemidesmosomes of the epidermis, suggesting its role in epidermal cell-basement membrane adhesion. The clone 439-9B was clustered as CD104 at the fifth Human Leucocyte Differentiation Antigen International Workshop. It may be used for immunoprecipitation, immunoblotting and immunohistochemistry on frozen tissue sections. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Profile of A431, an epidermal carcinoma cell line, analyzed on a FACScan
BD Pharmingen™ Purified Rat Anti-Human CD104 - Purified - Clone 439-9B - Isotype Rat IgG2b, κ - Reactivity Hu - 0.1 mg
纯化方法
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C
储存方法
Store undiluted at 4°C.
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Sonnenberg, Calafat, Janssen, Daams, van der Raaij-Helmer, Falcioni, Kennel, Aplin, Baker, Loizidou: "Integrin alpha 6/beta 4 complex is located in hemidesmosomes, suggesting a major role in epidermal cell-basement membrane adhesion." in: The Journal of cell biology, Vol. 113, Issue 4, pp. 907-17, (1991) (PubMed).
Tamura, Rozzo, Starr, Chambers, Reichardt, Cooper, Quaranta: "Epithelial integrin alpha 6 beta 4: complete primary structure of alpha 6 and variant forms of beta 4." in: The Journal of cell biology, Vol. 111, Issue 4, pp. 1593-604, (1990) (PubMed).
Hemler, Crouse, Sonnenberg: "Association of the VLA alpha 6 subunit with a novel protein. A possible alternative to the common VLA beta 1 subunit on certain cell lines." in: The Journal of biological chemistry, Vol. 264, Issue 11, pp. 6529-35, (1989) (PubMed).