The 13-11 monoclonal antibody specifically recognizes Troponin T type 2 (cardiac), encoded by the gene TNNT2. Troponin T is the tropomyosin-binding subunit of the troponin complex, which also encompasses troponin C and troponin I. This complex regulates muscle contraction in skeletal and cardiac muscle in response to alterations in calcium levels. Troponin T type 2 is solely found in the heart, and genetic alterations in the TNNT2 gene are associated to a series of heart disorders in humans, including hypertrophic cardiomyopathy, dilated cardiomyopathy and left ventricular noncompaction. Cardiac Troponin T can be used as a marker for the identification of cardiomyocytes derived from pluripotent stem cells. LEFT PANEL: Western blot analysis of Cardiac Troponin T expression in human and mouse tissues. Human heart lysate was obtained from Abcam (Cat .No. ab2943, lane 1). C2C12 mouse myoblasts (ATCC CRL-1772) were cultured in DMEM containing 10 % FBS (lane 2) or 0.5 % FBS for 14 days for induction of cell differentiation (lane 3). Lysates were prepared, electrophoresed (SDS-PAGE) and transferred to PVDF membranes. The membranes were labeled with 1 μg/mL of Purified Mouse Anti-Cardiac Troponin T. Specific staining was detected with HRP Goat Anti-Mouse Ig (Cat. No. 554002). RIGHT PANEL: Immunohistochemical staining of Cardiac Troponin T in human heart. Following antigen retrieval with BD Retrievagen A Buffer (Cat. No. 550524), the formalin-fixed paraffin-embedded sections were stained with either Purified Mouse IgG1 κ Isotype Control (Cat. No.550878, left) or Purified Mouse Anti-Cardiac Troponin T (right). A three-step staining procedure that employs Biotin Goat Anti-Mouse Immunoglobulin (Cat. No. 550337), Streptavidin HRP (Cat. No.550946), and the DAB Substrate Kit (Cat. No. 550880) was used to develop the primary staining reagents. Original magnification: 40x. Flow cytometric analysis of Cardiac Troponin T in human (left panel) and mouse (right panel) cardiomyocytes differentiated in vitro. Human embryonic stem cell-derived cardiomyocytes (Evans lab, UCSD) were disassociated and fixed in BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with BD Phosflow™ Perm/Wash buffer I (Cat. No. 557885). The C2C12 mouse myoblast cell line (ATCC CRL-1772) was cultured for 5 days in low-serum conditions for induction of cell differentiation, fixed with BD Cytofix™ Fixation Buffer, and permeabilized with BD Perm/Wash™ Buffer (Cat. No. 554723). The cells were stained with either Purified Mouse IgG1, κ Isotype Control (Cat. No. 554121, dashed line) or Purified Mouse Anti-Cardiac Troponin T (solid line). The second-step reagents were FITC Goat Anti-Mouse Ig (Cat. No. 554001, left) and PE Goat Anti-Mouse Ig (Cat. No. 550589, right). Events with the forward and side light-scatter characteristics of intact cells were gated. Flow cytometry was performed on a BD LSR Fortessa™ (left) or a BD FACSCanto™ II (right) flow cytometry system.
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储存条件
4 °C
储存方法
Store undiluted at 4°C.
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抗原
Cardiac Troponin T2 (cTnT)
(Cardiac Troponin T (cTnT))