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Rpb1 CTD 抗体 (pSer5, Ser5)

适用: 人 WB, IF, ICC, ChIP, ChIP-seq, IP 宿主: 大鼠 Monoclonal 3E8 unconjugated
产品编号 ABIN2668541
发货至: 中国
  • 抗原 See all Rpb1 CTD products
    Rpb1 CTD (RNA Polymerase II CTD)
    抗原表位
    • 8
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    pSer5, Ser5
    适用
    • 23
    • 6
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    宿主
    • 10
    • 9
    • 4
    大鼠
    克隆类型
    • 14
    • 9
    单克隆
    标记
    • 23
    This Rpb1 CTD antibody is un-conjugated
    应用范围
    • 23
    • 17
    • 14
    • 9
    • 6
    • 4
    • 3
    • 3
    • 2
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq), Immunoprecipitation (IP)
    纯化方法
    None
    免疫原
    This RNA pol II CTD pSer5 antibody was raised against a peptide containing the RNA pol II CTD sequence phosphorylated at serine 5.
    克隆位点
    3E8
    亚型
    IgG2a
  • 应用备注
    Optimal working dilution should be determined by the investigator.
    限制
    仅限研究用
  • 浓度
    1 μg/μL
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项

    Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

    储存条件
    -80 °C
    储存方法
    Antibodies in solution can be stored at -80 °C for 2 years.
    有效期
    6 months
  • Harlen, Trotta, Smith, Mosaheb, Fuchs, Churchman: "Comprehensive RNA Polymerase II Interactomes Reveal Distinct and Varied Roles for Each Phospho-CTD Residue." in: Cell reports, Vol. 15, Issue 10, pp. 2147-2158, (2016) (PubMed).

    Ramos, Wijetunga, McLellan, Suzuki, Greally: "DNA demethylation by 5-aza-2'-deoxycytidine is imprinted, targeted to euchromatin, and has limited transcriptional consequences." in: Epigenetics & chromatin, Vol. 8, pp. 11, (2015) (PubMed).

    Eifler, Shao, Bartholomeeusen, Fujinaga, Jäger, Johnson, Luo, Krogan, Peterlin: "Cyclin-dependent kinase 12 increases 3' end processing of growth factor-induced c-FOS transcripts." in: Molecular and cellular biology, Vol. 35, Issue 2, pp. 468-78, (2014) (PubMed).

    Hintermair, Heidemann, Koch, Descostes, Gut, Gut, Fenouil, Ferrier, Flatley, Kremmer, Chapman, Andrau, Eick: "Threonine-4 of mammalian RNA polymerase II CTD is targeted by Polo-like kinase 3 and required for transcriptional elongation." in: The EMBO journal, Vol. 31, Issue 12, pp. 2784-97, (2012) (PubMed).

    Chapman, Heidemann, Albert, Mailhammer, Flatley, Meisterernst, Kremmer, Eick: "Transcribing RNA polymerase II is phosphorylated at CTD residue serine-7." in: Science (New York, N.Y.), Vol. 318, Issue 5857, pp. 1780-2, (2007) (PubMed).

  • 抗原
    Rpb1 CTD (RNA Polymerase II CTD)
    别名
    RNA Pol II CTD (Rpb1 CTD 产品)
    分子量
    240 kDa
    基因ID
    5430
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