The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Stage-Specific Embryonic Antigen-3 (SSEA-3) is an internal core structure of glob-series glycolipids (R-Galβ 1-3GalNAcβ1-3Galα1-4Galβ1-R). SSEA-3 is expressed on human teratocarcinoma cells (EC), embryonic stemcells (ESC), embryonic germ cells (EG), and preimplantation embryos. The expression of SSEA-3 on human ESC is downregulated upon differentiation. SSEA-3 is also expressed by mouse on unfertilized eggs and preimplantation to early cleaved embryos.