我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

IL-6 Receptor 抗体 (PE-Cy7)

Name: IL-6 Receptor 抗体 (PE-Cy7)
SKU: ABIN2659081
Availability: OutOfStock
Rating: 5 (1 reviews)

IL6R 适用: 人 FACS 宿主: 小鼠 Monoclonal UV4 PE-Cy7

Flow Cytometry validation image for anti-Interleukin 6 Receptor (IL6R) antibody (PE-Cy7) (ABIN2659082) (IL-6 Receptor 抗体 (PE-Cy7))

FACS

1 image

(1 validation)

产品编号 ABIN2659081

发货至: 中国

Datasheet as PDF

抗原 See all IL-6 Receptor (IL6R) 抗体

IL-6 Receptor (IL6R) (Interleukin 6 Receptor (IL6R))
适用
50

17

16
人
宿主
47

23

5

1

1
小鼠
Compatible Secondaries
克隆类型
50

26

1
单克隆
标记
50

13

5

1

1

1

1

1

1

1

1

1
This IL-6 Receptor antibody is conjugated to PE-Cy7
应用范围
51

23

23

22

12

10

4

3

3

3

2

2

2

2

1

1
Flow Cytometry (FACS)

纯化方法

The antibody was purified by affinity chromatography and conjugated with PE/Cy7 under optimal conditions. The solution is free of unconjugated PE/Cy7 and unconjugated antibody.

克隆位点

UV4

亚型

IgG1 kappa

Top Product

Discover our top product IL6R Primary Antibody
anti-Interleukin 6 Receptor (IL6R) antibody
IL6R 适用: 人, 大鼠, 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Interleukin 6 Receptor (IL6R) (AA 20-320) antibody
IL6R 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Interleukin 6 Receptor (IL6R) (AA 216-356) antibody
IL6R 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Interleukin 6 Receptor (IL6R) antibody
IL6R 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Interleukin 6 Receptor (IL6R) (AA 1-468) antibody
IL6R 适用: 人 WB, FACS 宿主: 小鼠 Polyclonal unconjugated

anti-Interleukin 6 Receptor (IL6R) (AA 205-347) antibody
IL6R 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Interleukin 6 Receptor (IL6R) (AA 385-462) antibody
IL6R 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Interleukin 6 Receptor (IL6R) (AA 1-468) antibody
IL6R 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

anti-Interleukin 6 Receptor (IL6R) (AA 21-120) antibody
IL6R 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1E11 unconjugated

anti-Interleukin 6 Receptor (IL6R) (AA 21-120) antibody
IL6R 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2G6 unconjugated

应用备注

Optimal working dilution should be determined by the investigator.

限制

仅限研究用
生效 #101960 (Flow Cytometry)

by

Immunmodulatorische Abteilung, Universitätsklinikum Erlangen

No.

#101960

日期

2017.11.06

抗原

IL6R

Lot Number

B236171

Method validated

Flow Cytometry

Positive Control

Mature dendritic cells (mDCs) from three donors, incubated with ABIN2659082

Negative Control

Mature dendritic cells (mDCs) from three donors, incubated without antibody

Notes

Passed. ABIN2659082 specifically recognizes the IL-6 Receptor on mDCs.

Validation Images

A Flow cytometry analysis of mature DCs from three different donors after incubation for 30min or 120min with or without ABIN2659082. B Consecutive gating for single cells (left panel), mDCs (middle panel), and living cells (right panel), illustrated on donor 2 samples stained for 30min with ABIN2659082.
Full Methods
Primary Antibody

ABIN2659082

Secondary Antibody

Full Protocol

Prepare peripheral blood mononuclear cells (PBMCs) from leukoreduction system chambers (LRSCs) (Pfeiffer et al. (2013); Weidinger et al. 2011) from three healthy donors (obtained following informed consent and approved by the institutional review board) by density centrifugation on a Lymphoprep gradient (Axis-shield AS, 1114547, lot 12IFS10).
For the generation of dendritic cells (DCs), prepare PBMCs from leukoreduction system chambers (LRSC) (Pfeiffer et al. (2013)).
After centrifugation, collect PBMCs forming a distinct band at the sample/Lymphoprep interface.
Wash PBMCs 3x with cold PBS supplemented with 1mM EDTA.
Wash PBMCs 1x with cold RPMI 1640 (Lonza, BE12-167F, lot 7MB088).
Resuspend PBMCs in warm DC Medium (RPMI 1640 supplemented with 1% AB-serum, 100U/ml penicillin, 100mg/ml streptomycin, 2mM L-glutamine, 10mM HEPES buffer (Lonza, BE17-737E)) and seeded into 175cm² tissue culture flasks at a density of 3.5x10⁸ cells/flask.
Let monocytes adhere for 1h at 37°C and 5% CO₂.
Wash non-adherent fraction off with warm RPMI 1640.
Add 30ml DC medium supplemented with 800U/ml recombinant human granulocyte-macrophage colony-stimulating factor (GM-CSF) and 250U/ml recombinant human interleukin-4 (IL-4) to each flask (day 1).
Incubate cells for 72h at 37°C and 5% CO₂.
On day 4 (or three days later), add 5ml of fresh culture medium containing GM-CSF (final concentration of 400 U/ml in 35ml) and IL-4 (final concentration of 250U/ml in 35ml) to the cells.
On day 5, mature the immature DCs by adding 10ng/ml recombinant human tumor necrosis factor alpha (TNF-α), 1μg/ml prostaglandin E2 (PGE2), 200U/ml recombinant human interleukin-1β (IL-1β), 1000U/ml recombinant human interleukin-6 (IL-6), 40U/ml GM-CSF, and 250 U/ml IL-4 to the medium.
Incubate cells for 48h at 37°C and 5% CO₂.
On day 7, use the now mature DCs (mDCs) for FACS analysis.
Analyze cell surface expression of IL-6 Receptor by FACS:
Harvest 2.5x10⁵ mDCs by centrifugation.
Wash cells once with FACS buffer (PBS supplemented with 2% FCS).
Combine anti-human PE-Cy7 conjugated IL-6 Receptor antibody (antibodies-online, ABIN2659082, lot B236171) diluted 1:100 and LIVE/DEAD Violet dead cell stain kit (ThermoFisher Scientific, L34955, lot 1832693) diluted 1:300 in cold FACS buffer.
Resuspend mDCs in 100µl of the antibody-solution and incubate for 30min on ice in the dark.
Wash mDCs 2x with cold FACS buffer.
Fix mDCs using 2% paraformaldehyde in cold PBS.
Store mDCs at 4°C until analysis.
Run samples using a BD FACS Canto II and analyze events using FCS Express 5.

Experimental Notes

Mature DCs were stained for FACS for the analysis of the expression of IL-6 Receptor/CD126 on the cell surface of the cells with ABIN2659082 for 30min or 120min respectively. The histogram shows a clearly shift between unstained control and the stained mock control by staining for 120min. The staining for 30min does also show a shift of the histogram peak, but not as clear as the staining for 120min.
缓冲液

Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Protect from prolonged exposure to light. Do not freeze.

储存条件

4 °C

储存方法

The antibody solution should be stored undiluted between 2°C and 8°C.
抗原

IL-6 Receptor (IL6R) (Interleukin 6 Receptor (IL6R))

别名

CD126 (IL6R 产品)

别名

IL6R antibody, il-6ra antibody, CD126 antibody, IL-6R-1 antibody, IL-6RA antibody, IL6Q antibody, IL6RA antibody, IL6RQ antibody, gp80 antibody, IL6R1 antibody, Il6ra antibody, IL-6Ralpha antibody, IL-6R antibody, Il6r antibody, interleukin 6 receptor antibody, interleukin 6 receptor, alpha antibody, IL6R antibody, il6r antibody, Il6r antibody, Il6ra antibody

背景

CD126 is an 80 kD IL-6 receptor α chain also known as IL-6R. It is a member of the immunoglobulin superfamily that is expressed on plasma cells, T cells, activated B cells, monocytes, granulocytes, hepatocytes, epithelial cells, and fibroblasts. Functional IL-6 receptors are formed by the non-covalent association of CD126 and the IL-6 receptor β chain (CD130 or gp130). CD126 binds IL-6 with low affinity but does not signal. The β chain (gp130, CD130) does not bind IL-6 by itself but associates with the α-chain/IL-6 complex to initiate signal transduction. IL-6 binding to the receptor complex results in the stimulation of B and T cells, and hematopoietic precursor proliferation and differentiation. A soluble form of CD126 has been found in human serum.

途径

JAK/STAT Signaling, Autophagy, Growth Factor Binding, Cancer Immune Checkpoints