Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)
纯化方法
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
克隆位点
2-11
亚型
IgG
应用备注
Optimal working dilution should be determined by the investigator.
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Protect from prolonged exposure to light. Do not freeze.
储存条件
4 °C
储存方法
The antibody solution should be stored undiluted between 2°C and 8°C.
抗原
TCR V Gamma1.1/Cr4
背景
T cell receptor (TCR) is a heterodimer consisting of an α and β chain (TCR α/β) or a γ and δ chain (TCR γ/δ). TCR associates with CD3 to form a CD3/TCR complex. The CD3/TCR plays a key role in antigen recognition, signal transduction, and T cell activation. TCR Vγ1.1 (Garman nomenclature) is also called TCR Vγ1 (Tonegawa nomenclature). The Vγ1 gene almost exclusively rearranges to the Jγ4-Cγ 4 gene. Vγ1- Jγ4-Cγ4 expressing cells constitute a major population of γ/δ T cells in thymus and peripheral lymphoid organs in adult mice, but they are only composed of a minor population of γ/δ T cells during fetal and early postnatal life. Vγ1 T cell development can happen in thymus-dependent and thymus-independent manners. Further studies have shown that the antibody 2.11 recognized epitote is located in Cr4 domain.