Western Blot (5-10 μg/mL). Positive control: ovary. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
缓冲液
Phosphate buffered saline with 0.08 % sodium azide
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
-20 °C
储存方法
Store the product (in aliquots) at -20 °C. Can be shipped at 2 - 8 °C.
抗原
COL4A3BP
(Collagen, Type IV, alpha 3 (Goodpasture Antigen) Binding Protein (COL4A3BP))
COL4A3BP antibody, 2810404O15Rik antibody, 9230101K08Rik antibody, AU016711 antibody, CERT antibody, GPBP antibody, col4a3bp antibody, wu:fc37f11 antibody, zgc:92147 antibody, CERTL antibody, STARD11 antibody, cert antibody, certl antibody, cetp antibody, gpbp antibody, stard11 antibody, collagen type IV alpha 3 binding protein antibody, collagen, type IV, alpha 3 (Goodpasture antigen) binding protein antibody, collagen, type IV, alpha 3 (Goodpasture antigen) binding protein a antibody, collagen, type IV, alpha 3 (Goodpasture antigen) binding protein L homeolog antibody, COL4A3BP antibody, Col4a3bp antibody, col4a3bpa antibody, col4a3bp.L antibody
背景
CERT mediates the ATP-dependent ER-to-Golgi transfer of ceramide in a non-vesicular manner. The biosynthesis of lipids involves steps that occur in different intracellular compartments. The movement of lipids within these compartments is important in lipid-mediated signalling. Human CERT is identical to a splice variant of human Goodpasture antigen-binding protein (GPBP26). CERT contains a phosphoinositide-binding pleckstrin-homology (PH) domain (which targets CERT to the Golgi by binding phosphatidylinositol-4-phosphate (PtdIns4P)), a middle region, and a putative lipid-transfer-catalysing domain called START. CERT and CERTL can specifically extract ceramide from phospholipid bilayers in a START-domain-dependent manner. CERT interacts with ER membranes and specifically extracts ceramide. CERT catalyses both the specific extraction of ceramide from donor vesicles and its transfer to acceptor vesicles. CERT can associate with the Golgi in a PtdIns4P dependent manner.Synonyms: CERT, Ceramide transfer protein, Collagen type IV alpha-3-binding protein, GPBP, Goodpasture antigen-binding protein, STARD11, START domain-containing protein 11, StAR-related lipid transfer protein 11, StARD11, hCERT