Western blot: (1: 400 starting dilution). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
缓冲液
Phosphate buffered saline with 0.08 % sodium azide
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
-20 °C
储存方法
Store the product (in aliquots) at -20 °C. Can be shipped at 2 - 8 °C.
Activation of Sphingosine kinase (Sphk) increases the bioactive lipid, sphingosine 1-phosphate (S1P) and has been observed in a variety of cancer cells. Phosphorylation of sphingosine by sphingosine kinase (Sphk) is the rate-limiting step in the cellular synthesis of S1P. A principle activation event is the phosphorylation of serine 225 of sphiongosine kinase 1. Inhibition of Sphk1 phosphorylation and hence, Sphk activity is an important target for the development of drugs for cancer, inflammation, cardioprotection as well as a many other indications.Synonyms: SPHK, SPK, SPK 1, SPK-1