Western Blot: 1 - 10 μg/mL. Immunofluorescence. Immunoprecipitation. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
缓冲液
Phosphate buffered saline with 0.08 % sodium azide
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
-20 °C
抗原
BAPTA
物质类
Chemical
背景
BAPTA (bis(o-aminophenoxy)-ethane-N,N,N',N'-tetraacetic acid)is a calcium channel chelator. BAPTA has spawned many of the most familiar fluorescent indicators for calcium and other ions (1).BAPTA and its analogs including BAPTA-AM have proved most critical in cell physiology where they have been essential in defining the mechanisms underlying intra or intercellular calcium homeostasis (2).and calcium dependant exocytosis (3).BAPTA and its analogs may also find application in diseases which a disturbed regulation of excitatory neurotransmitter release and uptake plays a role (4). The rabbit BAPTA antibody recognizes a 69 kDa monomer and 138 kDa dimer by western blot. The immunogen used was a recombinant BAPTA-KLH.Synonyms: N, N', N'-tetraacetic acid, bis(o-aminophenoxy)-ethane-N, calcium channel chelator