Detects 23 kDa (Human) and 19 kDa (Other species) proteins, corresponding to the molecular mass of Cu/Zn superoxide dismutase (SOD) on SDS-PAGE immunoblots.
Western blot (6,8): 0.2 μg/mL was sufficient for detection of Cu/Zn SOD in 20 g of HeLacell lysate. Immunoprecipitation (8). EIA (6). Immunohistochemistry on frozen sections(7). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
状态
Liquid
浓度
1.0 mg/mL
缓冲液
PBS ( pH 7.0), 0.09 % Sodium Azide, 50 % Glycerol
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2¯ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2,5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extracellular space(9). SOD3 can also be distinguished by its heparin-binding capacity (1).Synonyms: ALS1, CuZn-SOD, CuZnSOD, IPOA, SOD-1, Superoxide dismutase [Cu-Zn]