Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
交叉反应 (详细)
Histone-H-1 100%, No reaction with Histone-H-2b, Histone-H-3 or Histone-H-4Very low crossreaction with Histone-H-core
纯化方法
purified
免疫原
Histone-1 antibody was raised in sheep using Intact calf histone H1 complexed with RNA. as the immunogen.
应用备注
IF: 1:100, IP: 1:20, WB: 1:300 Optimal conditions should be determined by the investigator.
限制
仅限研究用
浓度
Lot specific
缓冲液
Protein G purified and supplied in PBS, pH 7.4 .
注意事项
Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use.
储存条件
-20 °C/-80 °C
储存方法
Aliquot and store at -20 °C for short term storage, -70 °C for long-term storage.
抗原
Histone 1
背景
Histones are highly alkaline proteins found in eukaryotic cell nuclei, which package and order the DNA into structural units called nucleosomes. They are the chief protein components of chromatin, acting as spools around which DNA winds, and play a role in gene regulation.