Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with asynthetic peptide derived from the Cterminal of the TCP-1β protein and mouse myelomaAg8563 cells. Sequence common in human, frog, mouse, rat.
Western Blot: 1 - 2 μg/mL. Immunohistochemistry on paraffin sections: 1 - 5 μg/mL. Positive Control: Colorectal cancer tissue, Calu6 and HT29 cell lysates. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
缓冲液
PBS, 0.08 % Sodium Azide
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
The antibody can be shipped at ambient temperature. Store (in aliquots) at -20 °C only. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
The protein TCP-1 (t-complex polypeptide 1) is a subunit of the hetero-oligomeric complex CCT (chaperonin containing TCP-1) present in the eukaryotic cytosol. The CCT of eukaryotic cytosol is composed of eight different subunit species that are proposed to have independent functions in folding its in vivo substrates, the Actins and Tubulins. TCP-1 was first identified in the mouse as relevant for tail-less and embryonic lethal phenotypes. Sequences homologous to TCP-1 have been isolated in several other species, and the yeast TCP-1 has been shown to encode a molecular chaperone for Actin and Tubulin. TCP-1 found in mammalian cells and yeast plays an important role in the folding of cytosolic proteins.Synonyms: 99D8.1, CCT-beta, CCTB, T-complex protein 1 subunit beta, TCP-1 beta