Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))
特异性
This antibody reacts to Heterogeneous Nuclear Ribonucleoprotein K.
产品特性
Synonyms: HNRPK, Heterogeneous nuclear ribonucleoprotein K, TUNP, Transformation up-regulatednuclear protein
纯化方法
Purified
免疫原
Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with asynthetic peptide derived from the C-terminal of the hnRNP-K protein and Mouse myelomaAg8563 cells. Remarks: Sequence is common in Human, Mouse, Rat, Rabbit and Chicken.
Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
缓冲液
PBS, 0.08 % Sodium Azide
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
The antibody can be shipped at ambient temperature. Store (in aliquots) at -20 °C only. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
有效期
12 months
抗原
HNRNPK
(Heterogeneous Nuclear Ribonucleoprotein K (HNRNPK))
Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of polypeptides that contribute to mRNA transcription and premRNA processing as well as mature mRNA transport to the cytoplasm and translation. They also bind heterogeneous nuclear RNA (hnRNA), which are the transcripts produced by RNA Polymerase II. There are approximately 20 known hnRNP proteins, which range in size from 34 kDa to 120 kDa, and their complexes are the major constituents of the spliceosome. The majority of hnRNP proteins components are localized to the nucleus, however some shuttle between the nucleus and the cytoplasm, such as hnRNP K. hnRNP K, a 65 kDa protein, recruits a variety of molecular partners through two K homologous (KH) domains, which are required for protein-protein interactions. hnRNP K also contains several potential phosphorylation sites, including Ser 302, the major site of PKCd phosphorylation, which are thought to regulate various cellular functions, including sequence-specific DNA binding, transcription, RNA binding and nucleocytoplasmic shuttling.Synonyms: HNRPK, Heterogeneous nuclear ribonucleoprotein K, TUNP, Transformation up-regulated nuclear protein