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Nitrotyrosine 抗体

WB, IHC (fro) 宿主: 小鼠 Monoclonal 2A12 unconjugated
产品编号 ABIN264465
发货至: 中国
  • 抗原 See all Nitrotyrosine 抗体
    Nitrotyrosine
    适用
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    宿主
    • 25
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    小鼠
    克隆类型
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    单克隆
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    This Nitrotyrosine antibody is un-conjugated
    应用范围
    • 49
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    Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))
    产品特性
    Synonyms: NO-Tyrosine, Nitro-Tyrosine
    纯化方法
    Purified
    免疫原
    3-Nitrotyrosine-KLH
    克隆位点
    2A12
    亚型
    IgG1
    Top Product
    Discover our top product Nitrotyrosine Primary Antibody
  • 应用备注
    Western Blot: 1/1000. Immunohistochemistry on Frozen Sections.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    限制
    仅限研究用
  • 缓冲液
    20 mM Sodium Phosphate, 150 mM Sodium Chloride, 50 % Glycerol, 3 mM Sodium Azide, pH 7.5
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    -20 °C
    储存方法
    The antibody can be shipped at 2-8 °C. Store (in aliquots) at -20 °C only. Avoid repeated freezing and thawing.
    Shelf life: one year from despatch.
    有效期
    12 months
  • 抗原
    Nitrotyrosine
    Abstract
    Nitrotyrosine 产品
    物质类
    Chemical
    背景
    Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxidesignaling. While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase, myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Various studies have shown that protein tyrosinenitration is limited to specific proteins and that the process is selective. For example, exposure of human surfactant protein A (SP-A) to oxygen-nitrogen intermediates generated by activated alveolar macrophages resulted in specific nitration of SP-A at tyrosines 164 and 166, while addition of 1.2 mMCO 2 resulted in additional nitration at tyrosine 161. The presence of nitrotyrosine-containing proteins has shown high correlation to disease states such as atherosclerosis, Alzheimer's disease, Parkinson's disease and amyotrophic lateral sclerosis.55 kD 160 kD.Synonyms: NO-Tyrosine, Nitro-Tyrosine
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