HLA-DR 抗体 (FITC)
-
- 抗原 See all HLA-DR products
- HLA-DR
-
适用
- 人, 猴
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- This HLA-DR antibody is conjugated to FITC
-
应用范围
- Flow Cytometry (FACS)
- 产品特性
- Synonyms: HLA-DR, HLA class II histocompatibility antigen DR, MHC class II antigen DR
- 克隆位点
- 423L
- 亚型
- IgG2a
-
-
- 应用备注
-
Flow Cytometry. Monitoring of B cells in peripheral blood. Analysis of B subsets. Study of B cell activation. Study of B cell neoplasms.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 实验流程
- PBMC: Add 10 ul of MAB/10e6 PBMC in 100 ul PBS. Mix gently and incubate for 15 minutes at 2-8°C. Wash twice with PBS and analyze or fix with 0. 5% v/v of paraformaldehyde in PBS andanalyze. WHOLE BLOOD: Add 10 ul of MAB/100 ul of whole blood. Mix gently and incubate for 15 minutes at room temperature (20°C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0. 5% v/v of paraformaldehyde in PBS andanalyze. See instrument manufacturers instructions for Lysed Whole Blood andImmunofluorescence analysis with a flow cytometer or microscope.
- 限制
- 仅限研究用
-
- 缓冲液
- PBS containing 0.2 % protein carrier and 0.08 % Sodium Azide as preservative.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
-
Store the antibody undiluted at 2-8 °C. Do Not Freeze!
Shelf life: One year from despatch. - 有效期
- 12 months
-
- 抗原
- HLA-DR
- 别名
- HLA Class II DR (HLA-DR 产品)
- 背景
- Identification of human B cell and T cell subsets associated with approximately 10 % of peripheral blood lymphocytes 28-34,000 M.W. surface antigen, also low density on monocytes and macrophages.Synonyms: HLA class II histocompatibility antigen DR, HLA-DR, MHC class II antigen DR
- 途径
- Human Leukocyte Antigen (HLA) in Adaptive Immune Response
-