Flow Cytometry. This antibody is suitable for Monitoring of T cells subsets in peripheral blood, Analysis of Bcell subsets, Characterization of subtypes of T cell leukemias and lymphomas. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
实验流程
PBMC: Add 10 ul of Antibody/10e6 PBMC in 100 ul PBS. Mix gently and incubate for 15 minutes at 2-8°C. Wash twice with PBS and analyze or fix with 0. 5% v/v of paraformaldehyde in PBS andanalyze. WHOLE BLOOD: Add 10 ul of Antibody/100 ul of whole blood. Mix gently and incubate for 15 minutes at room temperature 20°C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0. 5% v/v of paraformaldehyde in PBS andanalyze. See instrument manufacturer's instructions for Lysed Whole Blood andImmunofluorescence analysis with a flow cytometer or microscope.
限制
仅限研究用
缓冲液
PBS containing 0.2 % protein carrier and 0.08 % Sodium Azide as preservative.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C
储存方法
Store the antibody undiluted at 2-8 °C. Do Not Freeze! Shelf life: One year from despatch.
Identification of human helper/inducer T cells expressing the 67,000 M.W. surface antigen, 85 % peripheral blood lyymphocytes that form rosettes with sheep red blood cells (E+), and a small subset of B cells.Synonyms: CD5, LEU1, Lymphocyte antigen T1/Leu-1, T-cell surface glycoprotein CD5